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Cited by 108Fatty Acid Research and HealthEdible Oils Quality and AnalysisAquaculture Nutrition and Growth

Eicosapentaenoic acid and docosahexaenoic acid from fish oils: differential associations with lipid responses

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Leigh-Firbank, E.C., Minihane A.M.*, Leake, D.S., Wright, J.W., Murphy, M.C., Griffin, B.A., Williams, C.M.·2002·British Journal of Nutrition
DOI 10.1079/bjnbjn2002556
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Abstract

Fish-oil supplementation can reduce circulating triacylglycerol (TG) levels and cardiovascular risk. This study aimed to assess independent associations between changes in platelet eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and fasting and postprandial (PP) lipoprotein concentrations and LDL oxidation status, following fish-oil intervention. Fifty-five mildly hypertriacylglycerolaemic (TG 1.5-4.0 mmol/l) men completed a double-blind placebo controlled cross over study, where individuals consumed 6 g fish oil (3 g EPA+DHA) or 6 g olive oil (placebo)/d for two 6-week intervention periods, with a 12-week wash-out period in between. Fish-oil intervention resulted in a significant increase in the platelet phospholipid EPA (+491 %, P<0.001) and DHA (+44 %, P<0.001) content and a significant decrease in the arachidonic acid (-10 %, P<0.001) and gamma-linolenic acid (-24 %, P<0.001) levels. A 30 % increase in ex vivo LDL oxidation (P<0.001) was observed. In addition, fish oil resulted in a significant decrease in fasting and PP TG levels (P<0.001), PP non-esterified fatty acid (NEFA) levels, and in the percentage LDL as LDL-3 (P=0.040), and an increase in LDL-cholesterol (P=0.027). In multivariate analysis, changes in platelet phospholipid DHA emerged as being independently associated with the rise in LDL-cholesterol, accounting for 16 % of the variability in this outcome measure (P=0.030). In contrast, increases in platelet EPA were independently associated with the reductions in fasting (P=0.046) and PP TG (P=0.023), and PP NEFA (P=0.015), explaining 15-20 % and 25 % of the variability in response respectively. Increases in platelet EPA+DHA were independently and positively associated with the increase in LDL oxidation (P=0.011). EPA and DHA may have differential effects on plasma lipids in mildly hypertriacylglycerolaemic men.

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  • When 4 g/day IPE was given with statins, placebo-adjusted decreases were 22% for triglycerides, 5% for HDL cholesterol, 14% for non-HDL cholesterol, 9% for apolipoprotein B, and 6% for LDL cholesterol, and LDL particle size modestly increased.

    “When IPE (4 g/d) was administered with statins, there were placebo-adjusted decreases of 22% in triglycerides, 5% in HDL-C, 14% in non-HDL-C, 9% in apo B, and 6% in LDL-C, 39 and LDL particle size was modestly increased.”

    From Omega-3 Fatty Acids for the Management of Hypertriglyceridemia: A Science Advisory From the American Heart Association (2019) · cites this paper as [47]

    The Plasma lipids, glucose, insulin section reports lower triglycerides, and the Discussion describes a shift toward larger LDL particles. But this was a trial of 6 g/day fish oil (EPA plus DHA), not 4 g/day IPE with statins; the reported percentages do not match, HDL cholesterol changed little, and LDL cholesterol increased rather than decreased. Our copy gives no corresponding non-HDL cholesterol or apo B reductions. (LLM, conf=0.89)

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Authors
Leigh-Firbank, E. C., A.M.*, M., Leake, D. S., Wright, J. W., Murphy, M. C., Griffin, B. A., & Williams, C. M.
Affiliations
  • University of Reading (GB)
  • University of Surrey (GB)
ORCID
Leake, D.S.
Venue
British Journal of Nutrition
Publisher
Cambridge University Press (CUP)
Published
2002-05-01
Volume
87
Issue
5
Pages
435-445
ISSN
  • 0007-1145 (ISSN-L)
  • 0000-0000
DOI
10.1079/bjnbjn2002556
OpenAlex
W2164880881
Cited by
108
References
0
Citations by year
2023: 2 · 2021: 1 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 8
FWCI
5.20
Type
journal article
Language
en
Open access
Locations
  • British Journal Of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
  • UEA Digital Repository (University of East Anglia) (submittedVersion)
Funders
  • Directorate for Biological Sciences
  • Biotechnology and Biological Sciences Research Council
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
eicosapentaenoic acid, docosahexaenoic acid, fish oil supplementation, platelet phospholipids, hypertriglyceridemia, LDL oxidation, LDL cholesterol, postprandial lipemia, free fatty acids, arachidonic acid, gamma-linolenic acid, olive oil, cardiovascular risk, plasma lipids, omega-3 fatty acids
MeSH terms
Blood Glucose, Blood Platelets, Docosahexaenoic Acids, Double-Blind Method, Fish Oils, Humans, Insulin, Lipids, Lipoproteins, Lipoproteins, LDL, Male, Middle Aged, Oxidation-Reduction, Regression Analysis, Eicosapentaenoic Acid, Cross-Over Studies, Postprandial Period, alpha-Tocopherol

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