The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A method for designing primer sets for speciation studies in filamentous ascomycetes
Abstract
A simple method is described for designing primer sets that can amplify specific protein-encoding sequences in a wide variety of filamentous ascomycetes. Using this technique, we successfully designed primers that amplified the intergenic spacer region of the nuclear ribosomal DNA repeat, portions of the translation elongation factor 1 alpha, calmodulin, and chitin synthase 1 genes, and two other genes encoding actin and ras protein. All amplicons were sequenced and determined to amplify the target gene. Regions were successfully amplified in Sclerotinia sclerotiorum and other sclerotiniaceous species, Neurospora crassa, Trichophyton rubrum, Aspergillus nidulans, Podospora anserina, Fusarium solani, and Ophiostoma novo-ulmi. These regions are a potentially rich source of characters for population and speciation studies in filamentous ascomycetes. Each primer set amplified a DNA product of predicted size from N. crassa.
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Cites
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
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Damm, U., Cannon, P. F., Woudenberg, J. H. C., & Crous, P. W. (2012) · Studies in Mycology · doi:10.3114/sim0010 · Cited by 979
Fungal diversity notes 111–252—taxonomic and phylogenetic contributions to fungal taxa
Ariyawansa, H. A., Hyde, K. D., Jayasiri, S. C., Buyck, B., Chethana, K. W. T., Dai, D. Q., Dai, Y. C., Daranagama, D. A., Jayawardena, R. S., Lücking, R., et al. (2015) · Fungal Diversity · doi:10.1007/s13225-015-0346-5 · Cited by 751
The Colletotrichum boninense species complex
Damm, U., Cannon, P. F., Woudenberg, J. H. C., Johnston, P. R., Weir, B. S., Tan, Y. P., Shivas, R. G., & Crous, P. W. (2012) · Studies in Mycology · doi:10.3114/sim0002 · Cited by 485
Slippers, B., Crous, P. W., Denman, S., Coutinho, T. A., Wingfield, B. D., & Wingfield, M. J. (2004) · Mycologia · doi:10.1080/15572536.2005.11833000 · Cited by 429
Metadata
- Authors
- Carbone, I., & Kohn, L. M.
- Venue
- Mycologia
- Publisher
- Informa UK Limited
- Published
- 1999-05-01
- Volume
- 91
- Issue
- 3
- Pages
- 553-556
- ISSN
- 0027-5514 (ISSN-L)
- 1557-2536
- OpenAlex
- W4230450401
- Cited by
- 4,217
- References
- 1
- FWCI
- 2.11
- Type
- journal article
- Language
- en
- Open access
- closed
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- Carbone, I., & Kohn, L. M. (1999). A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia. https://doi.org/10.1080/00275514.1999.12061051
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