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A Modified Hemoglobin Substrate Method for the Estimation of Pepsin in Gastric Juice

Berstad, A.·1970·Scandinavian Journal of Gastroenterology

Abstract

Berstad, A. 1970. A Modified Hemoglobin Substrate Method for the Estimation of Pepsin in Gastric Juice. Scand. J. Gastroent. 5, 343-348.Human hemoglobin was used as substrate for the estimation of pepsin, instead of bovine hemoglobin as used by Anson (1), becausc it is easily prepared and the sensitivity of the method encreased. In order to evaluate the method the influence of variation in substrate and enzyme concentration, time, temperature, and pH of incubation, trichloracetic acid concentration, and type of filter paper were investigated. The standard error of the method was ± 2.7%.

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Metadata

Authors
Berstad, A.
Venue
Scandinavian Journal of Gastroenterology
Published
1970-06-30
Volume
5
Issue
5
Pages
343-348
ISSN
  • 0036-5521 (ISSN-L)
  • 1502-7708
OpenAlex
W1424489583
Cited by
235
References
0
FWCI
9.73
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: A Modified Hemoglobin Substrate Method for the Estimation of Pepsin in Gastric Juice. https://app.cochat.ai/papers/10.1080/00365521.1970.12096601
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Berstad, A. (1970). A Modified Hemoglobin Substrate Method for the Estimation of Pepsin in Gastric Juice. Scandinavian Journal of Gastroenterology. https://doi.org/10.1080/00365521.1970.12096601

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.