Assessment of genetic fidelity of somatic embryogenesis regenerated guava ( Psidium guajava L.) plants using DNA‐based markers
Open accessAbstract
An efficient somatic embryogenesis protocol for plantlet regeneration of the commercially valuable, and nutritionally important, crop guava ( Psidium guajava L.) has been established. The immature zygotic embryos were targeted as an explant source for inducing somatic embryogenesis and plant regeneration, using four commercial cultivars (Allahabad Safeda, Lalit, Sardar and Shweta). The plantlets regenerated were assessed for genetic fidelity using random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and simple sequence repeat (SSR) molecular markers. A total of 2171 scorable bands were obtained using RAPD, ISSR or SSR, ranging from 300–3500, 250–3000 and 150–280 bp, respectively. No polymorphism was observed among the somatic embryogenesis regenerated plants, compared with respective donor mother plants. The profiles generated based on the three marker systems were found to be highly uniform and approximately 99% bands were monomorphic. This high degree of genetic uniformity (as assessed using the markers) in the somatic embryogenesis regenerated plants indicates genomic stability was maintained through the regeneration protocol. The somatic embryogenesis regenerated plants were hardened and transferred to the field for acclimatization, of which 80% plantlets survived, with all being phenotypically similar to the donor mother plants. We conclude that the RAPD, ISSR and SSR markers were informative and potentially useful in confirming the uniformity assessment of somatic embryogenesis regenerated plants.
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RAPD can sometimes yield inconsistent results and may not be as reproducible as other methods such as AFLP and SSR.
“Despite its simplicity and low cost, RAPD can sometimes yield inconsistent results and may not be as reproducible as other methods, such as AFLP and SSR [121,122].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [122]
The Introduction (page not available) describes RAPD as simple and cost-effective and says SSR markers can be more reliable. It does not compare RAPD with AFLP or explicitly say RAPD yields inconsistent results. The Statistical analysis section says the authors retained only consistently reproducible bands, but does not establish how often RAPD results are inconsistent. (LLM, conf=0.85)
Experimental — accuracy not yet measuredIn Psidium guajava, approximately 99% of bands were monomorphic.
“Approximately 99% of bands were monomorphic.”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [122]
The Molecular analysis section reports that all 1,323 RAPD bands were monomorphic across somatic-embryogenesis-regenerated guava plants compared with their mother plants. It does not report a 99% figure for the bands overall, so the claim’s percentage and scope are not fully supported. (LLM, conf=0.82)
Experimental — accuracy not yet measured
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- Authors
- Kamle, M., Kumar, P., Bajpai, A., Kalim, S., & Chandra, R.
- Affiliations
- Central Institute For Subtropical Horticulture (IN)
- Ben-Gurion University of the Negev (IL)
- Bundelkhand University (IN)
- Corresponding author
- Kamle, M
- Venue
- New Zealand Journal of Crop and Horticultural Science
- Publisher
- Wiley
- Published
- 2013-08-21
- Volume
- 42
- Issue
- 1
- Pages
- 1-9
- ISSN
- 0114-0671 (ISSN-L)
- 1175-8783
- OpenAlex
- W1988652350
- Cited by
- 23
- References
- 45
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 4
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- New Zealand Journal of Crop and Horticultural Science (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Keywords
- guava, somatic embryogenesis, genetic fidelity, plant regeneration, genome stability, RAPD markers, ISSR markers, microsatellite markers, acclimatization
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