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Silver Staining of Myelin by Means of Physical Development

Gallyas, F.·1979·Neurological Research

Abstract

For staining myelin with silver a physical development technique has been devised that can render visible the thinnest fibers in various animal species, including fishes and reptiles, even in the early phase of myelination and may be applied to both frozen and embedded materials. Its principle is as follows: Myelin can form and bind colloidal silver particles in a 0.1% ammoniacal silver nitrate solution of pH 7.5. The production of metallic silver by other tissue elements is suppressed by the sections pretreated with a 2:1 mixture of pyridine and acetic anhydride for 30 min. The colloidal silver particles bound in the myelin are enlarged to microscopic dimensions by a special physical developer.

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Metadata

Authors
Gallyas, F.
Venue
Neurological Research
Published
1979-01
Volume
1
Issue
2
Pages
203-209
ISSN
  • 0161-6412 (ISSN-L)
  • 1743-1328
OpenAlex
W2000038982
Cited by
935
References
0
FWCI
7.77
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Silver Staining of Myelin by Means of Physical Development. https://app.cochat.ai/papers/10.1080/01616412.1979.11739553
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Gallyas, F. (1979). Silver Staining of Myelin by Means of Physical Development. Neurological Research. https://doi.org/10.1080/01616412.1979.11739553

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.