Formulation, characterization and evaluation of rotavirus encapsulated PLA and PLGA particles for oral vaccination
Abstract
Polylactide (PLA) and polylactide-co-glycolide (PLGA) particles entrapping rotavirus (strain SA11) were formulated using a solvent evaporation technique. To minimize denaturation of viral antigen during the emulsification process, serum albumin was used as a stabilizer. Use of NaHCO(3) and sucrose during the primary emulsification step resulted in uniform stabilized particles entrapping rotavirus. Sonication during the primary emulsion and homogenization during the secondary emulsion process resulted in particles of sizes 2-8 microm, whereas nanoparticles were formed when sonication was used during both primary and secondary emulsion processes. Scanning electron and atomic force microscopy showed uniform pores and roughness throughout the polymer particle surface. Single dose oral immunization with 20 microg of antigen entrapped in PLA particles elicited improved and long-lasting IgA and IgG antibody titer in comparison to the soluble antigen. The study shows results illustrating the usefulness of polymeric microparticles as a potential oral delivery system for rotavirus vaccine.
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Oral immunomodulation with polymeric particulate carriers has been investigated for immunosuppressive drugs, viral and bacterial antigens, hormones, anticancer drugs, and allergens.
“Oral immunomodulation with polymeric particulate carriers has been investigated for a wide range of therapeutic applications, such as immuno suppressive drugs [42], viral and bacterial antigens [58,65], hormones [5,73], anticancer drugs [74], and allergens [75].”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [65]
Hydrophobicity of polymer microspheres and a rough surface increase attachment to APC surfaces and enhance antibody response.
“the hydrophobicity of polymer microspheres and a rough surface further increase the attachment to the surface of APCs [65], and enhance the antibody response [66].”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [65]
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Use of lectin-functionalized particles for oral immunotherapy
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- Authors
- Nayak, B., Panda, A. K., Ray, P., & Ray, A. R.
- Affiliations
- All India Institute of Medical Sciences (IN)
- National Institute of Immunology (IN)
- Corresponding author
- Ray, Alok R.
- Venue
- Journal of Microencapsulation
- Publisher
- Informa UK Limited
- Published
- 2008-10-20
- Volume
- 26
- Issue
- 2
- Pages
- 154-165
- ISSN
- 0265-2048 (ISSN-L)
- 1464-5246
- OpenAlex
- W2121339524
- Cited by
- 57
- References
- 39
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 6 · 2015: 6 · 2014: 4 · 2013: 1 · 2012: 8
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microencapsulation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Council of Scientific and Industrial Research, India
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Agricultural and Biological Sciences › Food Science › Microencapsulation and Drying Processes
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Keywords
- rotavirus vaccine, polylactic acid, PLGA, oral vaccine delivery, microencapsulation, solvent evaporation, sonication, nanoparticle formation
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Administration, Oral, Animals, Cattle, Chemistry, Pharmaceutical, Circular Dichroism, Drug Compounding, Particle Size, Polyesters, Polyglycolic Acid, Polymers, Rotavirus, Serum Albumin, Bovine, Vaccination, Vaccines, Lactic Acid, Chitosan
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