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Optimisation of a cost-effective culture medium for the large-scale production of Bacillus subtilis UTB96
Abstract
Fermentation optimisation to achieve high biomass and high efficiency of a biocontrol product is millstone in biocontrol sciences. Here, a Placket–Burman design used for comparison and screening of some environmental factors that are effective on bacterial biomass of Bacillus subtilis UTB96. A response surface methodology used to determine the optimal points for three factors including pH, temperature and C/N ratio where the biomass is high. The results of response surface methodology application showed that the optimum conditions for maximum production of biomass in the medium occurs at pH 7, temperature 30 °C and C/N ratio of 23:1. Bacteria derived from optimised conditions either from laboratory or semi-industrial bioreactors, showed a considerable increase in biomass and also, their antagonistic activity against Phytophthora drechsleri in a plate assay. However, optimisation of culture medium in a laboratory bioreactor decreased the antagonistic activity against Aspergillus flavus. Application of the optimised culture medium in both semi-industrial and laboratory bioreactors reduced the length of the lag phase of bacterial growth.
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“A typical C:N ratio in B. subtilis cultivations is higher and in the range of 5:1 to 20:1 (Carvalho et al. 2010b; Ghasemi and Ahmadzadeh 2013; Monteiro et al. 2014).”
From Pilot-scale production of Bacillus subtilis MSCL 897 spore biomass and antifungal secondary metabolites in a low-cost medium (2024) · cites this paper as Ghasemi and Ahmadzadeh 2013
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Research works printed but not linked: 18
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Cited by
Sorted by citations
Bacillus Based Microbial Formulations: Optimization of the Production Process
Stamenkovic-Stojanovic, S., Karabegovic, I., Beskoski, V., Nikolic, N., & Lazic, M. (2019) · Hemijska industrija · doi:10.2298/hemind190214014s · Cited by 37
Bolmanis, E., Grigs, O., Didrihsone, E., Senkovs, M., & Nikolajeva, V. (2024) · Biotechnology Letters · doi:10.1007/s10529-024-03481-4 · Cited by 5
“A typical C:N ratio in B. subtilis cultivations is higher and in the range of 5:1 to 20:1 (Carvalho et al. 2010; Ghasemi and Ahmadzadeh 2013; Monteiro et al. 2014).”
Gracious, M., & Nandakumar, S. (2024) · Journal of Applied Biology & Biotechnology · doi:10.7324/jabb.2024.177031 · Cited by 2
Metadata
- Authors
- Ghasemi, S., & Ahmadzadeh, M.
- Affiliations
- University of Tehran (IR)
- Corresponding author
- Ahmadzadeh, Masoud
- ORCID
- Ahmadzadeh, Masoud
- Venue
- Archives Of Phytopathology And Plant Protection
- Publisher
- Informa UK Limited
- Published
- 2013-08
- Volume
- 46
- Issue
- 13
- Pages
- 1552-1563
- ISSN
- 0323-5408 (ISSN-L)
- 1477-2906
- OpenAlex
- W2086441823
- Cited by
- 13
- References
- 0
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2016: 1
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Archives of Phytopathology and Plant Protection (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Agriculture, Plant Science, Crop Management
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Entomopathogenic Microorganisms in Pest Control
- Keywords
- culture medium optimization, fermentation conditions, Plackett-Burman design, response surface methodology, C/N ratio, pH, temperature, bacterial biomass, biological control agents, Phytophthora drechsleri, Aspergillus flavus, antagonistic activity, lag phase
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- The paper
- Ghasemi, S., & Ahmadzadeh, M. (2013). Optimisation of a cost-effective culture medium for the large-scale production of Bacillus subtilis UTB96. Archives Of Phytopathology And Plant Protection. https://doi.org/10.1080/03235408.2013.771469
