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Cited by 14Pesticide Exposure and ToxicityNeuroscience and Neuropharmacology ResearchAnesthesia and Neurotoxicity Research

Chlorpyrifos-induced dysregulation of synaptic plasticity in rat hippocampal neurons

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Zhou, Wenjuan, Zhang, Chen, Wang, Peipei, Deng, Yuanying, Dai, Hongmei, Tian, Jing, Wu, Guojiao, Zhao, Lingling·2023·Journal of Environmental Science and Health, Part B
DOI 10.1080/03601234.2023.2171236
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Abstract

Chlorpyrifos (CPF) is a widely used organophosphorus pesticide. Increasing evidence has shown that exposure to CPF in early life might induce neurodevelopmental disorders, but the pathogenesis remains uncertain. Synaptic plasticity plays a crucial role in neurodevelopment. This study aimed to investigate the effect of CPF on synaptic plasticity in hippocampal neurons and establish the cellular mechanism underlying these effects. Using CPF-exposed rat and primary hippocampal neurons model, we analyzed the impact of CPF on the synaptic morphology, the expression level of a presynaptic protein, a postsynaptic protein and ionotropic glutamate receptors (iGluRs), as well as the effects on the Wnt/β-catenin pathway. We found that the synapses were shortened, the spines were decreased, and the expression of synaptophysin (Syp), postsynaptic density-95 (PSD-95), GluN1, GluA1 and Wnt7a, as well as active β-catenin in primary hippocampal neurons was decreased. Our study suggests that CPF exposure induced dysregulation of synaptic plasticity in rat hippocampal neurons, which might provide novel information regarding the mechanism of CPF-induced neurodevelopmental disorders.

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  • Chlorpyrifos exposure dysregulates synaptic plasticity in rat hippocampal neurons, reducing dendritic spine density and decreasing expression of synaptophysin, PSD-95, GluN1, and GluA1.

    “For example, chlorpyrifos exposure dysregulates synaptic plasticity in rat hippocampal neurons, reducing dendritic spine density and decreasing expression of synaptophysin, PSD-95, GluN1, and GluA1.”

    From Environmental endocrine disruptors at the synapse: mechanisms linking chemical exposure to cognitive and behavioral dysfunction (2026) · cites this paper as Zhou et al., 2023

  • Pesticides are not only general neurotoxicants but can also interfere directly with the molecular and synaptic processes that support neural plasticity.

    “Together, these findings indicate that pesticides are not only general neurotoxicants, but can also interfere directly with the molecular and synaptic processes that support neural plasticity (Bergman et al., 2012; Zhou et al., 2023).”

    From Environmental endocrine disruptors at the synapse: mechanisms linking chemical exposure to cognitive and behavioral dysfunction (2026) · cites this paper as Zhou et al., 2023

  • In mouse cerebral cortex, tri-, di-, and monobutyltin altered cholinergic synaptic parameters relevant to neurotransmission.

    “Organotin compounds can also directly impair synaptic communication: in mouse cerebral cortex, tri-, di-, and monobutyltin altered cholinergic synaptic parameters relevant to neurotransmission.”

    From Environmental endocrine disruptors at the synapse: mechanisms linking chemical exposure to cognitive and behavioral dysfunction (2026) · cites this paper as Zhou et al., 2023

    The Abstract and Discussion (pages unavailable) describe chlorpyrifos exposure and synaptic plasticity in rat hippocampal neurons, not butyltin effects in mouse cerebral cortex. Searches for tri-, di-, and monobutyltin, organotin, and cholinergic synaptic parameters found no passage addressing the attributed finding. (LLM, conf=0.76)

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Cited by

Sorted by citations

Authors
Zhou, W., Zhang, C., Wang, P., Deng, Y., Dai, H., Tian, J., Wu, G., & Zhao, L.
Affiliations
Central South University (CN)
Corresponding author
Zhao, Lingling
ORCID
Venue
Journal of Environmental Science and Health, Part B
Publisher
Informa UK Limited
Published
2023-02-01
Volume
58
Issue
2
Pages
100-109
ISSN
  • 0360-1234 (ISSN-L)
  • 1532-4109
DOI
10.1080/03601234.2023.2171236
OpenAlex
W4318755474
Cited by
14
References
4
Citations by year
2026: 8 · 2025: 2 · 2024: 3 · 2023: 1
FWCI
2.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Environmental Science and Health Part B (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
the Project for Xiangya famous doctor in Central South University (2014[68])
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Pesticide Exposure and Toxicity
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
Keywords
chlorpyrifos, organophosphorus pesticides, synaptic plasticity, hippocampal neurons, neurodevelopmental disorders, synaptic morphology, dendritic spines, synaptophysin, PSD-95, GluN1, GluR1, ionotropic glutamate receptors, Wnt/β-catenin signaling, Wnt7a, pesticide neurotoxicity
MeSH terms
Animals, Chlorpyrifos, Hippocampus, Neuronal Plasticity, Neurons, Organophosphorus Compounds, Pesticides, beta Catenin, Rats

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