The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Production and extraction of extracellular lipase from the entomopathogenic fungus Isaria fumosoroseus(Cordycipitaceae; Hypocreales)
Abstract
Lipases are important cuticle degrading enzymes involved in the infection process of entomopathogens by hydrolysing the ester bonds of lipoproteins, fats and waxes present in the insect integument. Production of extracellular lipase by Isaria fumosoroseus (Cordycipitaceae; Hypocreales) isolate IF28.2 was investigated using different combinations of basal medium components. The effect of different vegetable oils added to a basal medium at different concentrations to improve enzyme production was evaluated. Maximum lipase activity (125.33±2.96 U/mL) as well as maximum biomass production (22.36±0.99 mg/mL) was observed for olive oil when used at a concentration of 2% (v/v) of the basal medium. In the presence of surfactants, the highest lipase activity occurred when SDS and Tween 80 were added at the time of fungal inoculation. SDS proved to be the best surfactant having 110.66±3.52 U/mL lipase activity. The effects of the divalent metal ions (iron and magnesium) on lipase activity were also studied. Iron inhibited, whereas magnesium slightly increased lipase activity. The optimum pH for lipase production was 5.7 while 32°C proved to be the best temperature for lipase production.
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“Since a number of studies have highlighted the role of lipases in the biocontrol efficacy of bacteria and fungi against plant diseases and pests (Ali et al. 2009; Berto et al. 2001; Beys da Silva et al. 2010a, b; Keyhani 2018; Manuel et al. 2012; Sánchez-Pérez et al. 2014; Vial et al. 2007; Zha et al. 2014), the lipolytic activity of antagonistic yeasts may represent a promising target for”
From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Ali et al. 2009
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Cited by
Biocontrol yeasts: mechanisms and applications
Freimoser, F. M., Rueda-Mejia, M. P., Tilocca, B., & Migheli, Q. (2019) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-019-2728-4 · Cited by 462
Journey of enzymes in entomopathogenic fungi
Mondal, S., Baksi, S., Koris, A. K., & Vatai, G. N. (2016) · Pacific Science Review A Natural Science and Engineering · doi:10.1016/j.psra.2016.10.001 · Cited by 153
Lipid biology in fungal stress and virulence: Entomopathogenic fungi
Keyhani, N. O. (2017) · Fungal Biology · doi:10.1016/j.funbio.2017.07.003 · Cited by 132
Dhawan, M. K., & Joshi, N. (2017) · Brazilian Journal of Microbiology · doi:10.1016/j.bjm.2016.08.004 · Cited by 83
Enzymes of Entomopathogenic Fungi, Advances and Insights
Pérez, L. D. C. S., Barranco-Florido, J. E., Rodríguez-Navarro, S., Cervantes-Mayagoitia, J. F., & Ramos-López, M. Á. (2014) · Advances in Enzyme Research · doi:10.4236/aer.2014.22007 · Cited by 88
Metadata
- Authors
- Ali, S., Huang, Z., & Ren, S. X.
- Affiliations
- South China Agricultural University (CN)
- Corresponding author
- Ren, Shun X.
- Venue
- Biocontrol Science and Technology
- Publisher
- Informa UK Limited
- Published
- 2009-01
- Volume
- 19
- Issue
- 1
- Pages
- 81-89
- ISSN
- 0958-3157 (ISSN-L)
- 1360-0478
- OpenAlex
- W2001182405
- Cited by
- 29
- References
- 28
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 4 · 2019: 2 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 3
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biocontrol Science and Technology (publishedVersion)
- Funders
- Shandong Academy of Sciences
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Entomopathogenic Microorganisms in Pest Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- extracellular lipase, entomopathogenic fungi, olive oil, vegetable oil supplementation, surfactants, sodium dodecyl sulfate, Tween 80, divalent metal ions, iron inhibition, magnesium, enzyme production
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- The paper
- Ali, S., Huang, Z., & Ren, S. X. (2009). Production and extraction of extracellular lipase from the entomopathogenic fungus Isaria fumosoroseus(Cordycipitaceae; Hypocreales). Biocontrol Science and Technology. https://doi.org/10.1080/09583150802588524
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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