Neuroprotective effects of food restriction in a rat model of traumatic brain injury - the role of glucocorticoid signaling
Abstract
Objective Traumatic brain injury (TBI) is one of the most common causes of neurological damage in young and middle aged people. Food restriction (FR) has been shown to act neuroprotectively in animal models of stroke and TBI. Indeed, our previous studies showed that FR attenuates inflammation, through suppression of microglial activation and TNF-α production, suppresses caspase-3-induced neuronal cell death and enhances neuroplasticity in the rat model of TBI. Glucocorticoids (GCs) play a central role in mediating both molecular and behavioral responses to food restriction. However, the exact mechanisms of FR neuroprotection in TBI are still unclear. The goal of the present study was to examine whether FR exerts its beneficial effects by altering the glucocorticoid receptor (GR) signaling alone and/or together with other protective factors. Methods To this end, we examined the effects of FR (50% of regular daily food intake for 3 months prior to TBI) on the protein levels of total GR, GR phosphoisoform Ser232 (p-GR) and its transcriptional activity, as well as 11β-HSD1, NFκB (p65) and HSP70 as factors related to the GR signaling. Results Our results demonstrate that FR applied prior to TBI significantly changes p-GR levels, and it's transcriptional activity during the recovery period after TBI. Moreover, as a pretreatment, FR modulates other protective factors in response to TBI, such as 11β-HSD1, NF-κB (p65) and HSP70 that act in parallel with GR in it's anti-inflammatory an…
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Dietary restriction leads to a more pronounced increase in SGK1 gene expression, creating a closer association between SGK1 expression and phosphorylated GR in rats on a restricted diet compared to those with ad libitum feeding.
“Dietary restriction leads to a more pronounced increase in SGK1 gene expression, creating a closer association between SGK1 expression and phosphorylated GR in rats on a restricted diet compared to those with ad libitum feeding”
From Serum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain (2024) · cites this paper as Perovic et al., 2022
Cites
Manders, E. M. M., Stap, J., Brakenhoff, G. J., DRIEL, R. V., & Aten, J. A. (1992) · Journal of Cell Science · doi:10.1242/jcs.103.3.857 · Cited by 739 · see citing works
Roles for NF-κB in nerve cell survival, plasticity, and disease
Mattson, M. P., & Meffert, M. K. (2006) · Cell Death and Differentiation · doi:10.1038/sj.cdd.4401837 · Cited by 597 · see citing works
Inflammatory Neurodegeneration and Mechanisms of Microglial Killing of Neurons
Brown, G. C., & Neher, J. J. (2010) · Molecular Neurobiology · doi:10.1007/s12035-010-8105-9 · Cited by 526 · see citing works
Molecular mechanisms of glucocorticoid action: what is important?
Newton, R. C. (2000) · Thorax · doi:10.1136/thorax.55.7.603 · Cited by 507 · see citing works
Plasma binding proteins as mediators of corticosteroid action in vertebrates
Breuner, C., & Orchinik, M. (2002) · Journal of Endocrinology · doi:10.1677/joe.0.1750099 · Cited by 457 · see citing works
Stress management – heat shock protein-70 and the regulation of apoptosis
Beere, H. M. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)01874-2 · Cited by 435 · see citing works
Axonal Damage in Traumatic Brain Injury
Smith, D. H., & Meaney, D. (2000) · The Neuroscientist · doi:10.1177/107385840000600611 · Cited by 372 · see citing works
Mechanisms of inflammatory neurodegeneration: iNOS and NADPH oxidase
Brown, G. C. (2007) · Biochemical Society Transactions · doi:10.1042/bst0351119 · Cited by 315 · see citing works
Mattson, M. P., Duan, W., & Guo, Z. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01586.x · Cited by 285 · see citing works
Effect of early nutrition on deaths due to severe traumatic brain injury.
Härtl, R., Gerber, L., Ni, Q.-H., & Ghajar, J. (2008) · Journal of Neurosurgery · doi:10.3171/jns/2008/109/7/0050 · Cited by 196 · see citing works
Traumatic brain injury: a review of pathophysiology and management
Sande, A. A., & West, C. (2010) · Journal of Veterinary Emergency and Critical Care · doi:10.1111/j.1476-4431.2010.00527.x · Cited by 161 · see citing works
Spinal cord injury-induced inflammation: A dual-edged sword
Bethea, J. R. (2000) · Progress in brain research · doi:10.1016/s0079-6123(00)28005-9 · Cited by 134 · see citing works
Mattson, M. P., Duan, W., Wan, R., & Guo, Z. (2004) · NeuroRx · doi:10.1602/neurorx.1.1.111 · Cited by 130 · see citing works
The glucocorticoid paradox of caloric restriction in slowing brain aging
PATEL, N., & Finch, C. E. (2002) · Neurobiology of Aging · doi:10.1016/s0197-4580(02)00017-9 · Cited by 115 · see citing works
Low, S. C., Chapman, K. E., Edwards, C. R. W., & Seckl, J. R. (1994) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0130167 · Cited by 108 · see citing works
Amsterdam, A., & Sasson, R. (2002) · Molecular and Cellular Endocrinology · doi:10.1016/s0303-7207(01)00722-5 · Cited by 106 · see citing works
The 70-kDa heat shock protein (Hsp70) as a therapeutic target for stroke
Kim, J. Y., Han, Y., Lee, J. E., & Yenari, M. A. (2018) · Expert Opinion on Therapeutic Targets · doi:10.1080/14728222.2018.1439477 · Cited by 103 · see citing works
Tanić, N., Perović, M., Mladenović, A., Ruždijić, S., & Kanazir, S. (2007) · Journal of Molecular Neuroscience · doi:10.1007/s12031-007-0006-7 · Cited by 103 · see citing works
Self-citationIntermittent Fasting Improves Functional Recovery after Rat Thoracic Contusion Spinal Cord Injury
Jeong, M., Plunet, W. T., Streijger, F., Lee, J. H. T., Plemel, J. R., Park, S., Lam, C. K., Liu, J., & Tetzlaff, W. (2011) · Journal of Neurotrauma · doi:10.1089/neu.2010.1609 · Cited by 84 · see citing works
Sasson, R., Tajima, K., & Amsterdam, A. (2001) · Endocrinology · doi:10.1210/endo.142.2.7942 · Cited by 71 · see citing works
Ghrelin protects against cell death of hippocampal neurons in pilocarpine-induced seizures in rats
Xu, J., Wang, S., Lin, Y. H., Cao, L., Wang, R., & Chi, Z. (2009) · Neuroscience Letters · doi:10.1016/j.neulet.2009.01.067 · Cited by 71 · see citing works
Lončarević-Vasiljković, N., Pešić, V., Todorović, S., Popić, J., Smiljanic, K., Milanović, D., Ruždijić, S., & Kanazir, S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037215 · Cited by 58 · see citing works
Self-citationJamieson, P. M., Fuchs, E., Flügge, G., & Seckl, J. R. (1997) · Stress · doi:10.3109/10253899709014743 · Cited by 52 · see citing works
Leakey, J. E. A., CHEN, S., Manjgaladze, M., Turturro, A., Duffy, P. H., Pipkin, J. L., & Hart, R. W. (1994) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1994.tb56828.x · Cited by 52 · see citing works
Guarnieri, D. J., Brayton, C. E., Richards, S., Maldonado‐Avilés, J. G., Trinko, R., Nelson, J. C., Taylor, J. R., Gourley, S. L., & Dileone, R. (2011) · Biological Psychiatry · doi:10.1016/j.biopsych.2011.06.028 · Cited by 51 · see citing works
The Role of Ghrelin in Neuroprotection after Ischemic Brain Injury
Spencer, S., Miller, A. A., & Andrews, Z. (2013) · Brain Science · doi:10.3390/brainsci3010344 · Cited by 40 · see citing works
Lončarević‐Vasiljković, N., Milanović, D., Pešić, V., Tešić, V., Brkić, M., Lazić, D., Avramović, V., & Kanazir, S. (2016) · Neurochemistry International · doi:10.1016/j.neuint.2016.02.017 · Cited by 34 · see citing works
Self-citationPelletier, G., Luu-The, V., Li, S., Bujold, G., & Labrie, F. (2007) · Neuroscience · doi:10.1016/j.neuroscience.2006.11.038 · Cited by 32 · see citing works
Lončarević‐Vasiljković, N., Pešić, V., Tanić, N., Milanović, D., Popić, J., Kanazir, S., & Ruždijić, S. (2009) · Experimental Neurology · doi:10.1016/j.expneurol.2009.08.024 · Cited by 26 · see citing works
Self-citationMabula, J. B., Chalya, P. L., Mchembe, M. D., Kihunrwa, A., Massinde, A. N., Chandika, A. B., & Gilyoma, J. M. (2012) · World Journal of Emergency Surgery · doi:10.1186/1749-7922-7-29 · Cited by 26 · see citing works
Dexamethasone exacerbates spatial acquisition deficits after traumatic brain injury in rats
Chen, X., Lin, Y., Wang, D., & Zhang, J. (2010) · Neurological Research · doi:10.1179/016164110x12681290831162 · Cited by 24 · see citing works
Lei, Q., Cui, X., Dong, W., Barrera, R., Nicastro, J. M., Coppa, G. F., Wang, P., & Wu, R. (2011) · Molecular Medicine · doi:10.2119/molmed.00390 · Cited by 23 · see citing works
Cell stress genes and neuronal protection
Latchman, D. S. (1995) · Neuropathology and Applied Neurobiology · doi:10.1111/j.1365-2990.1995.tb01089.x · Cited by 18 · see citing works
Intermittent Dietary Restriction as a Practical Intervention in Aging
Sharma, S., & Kaur, G. (2007) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1396.031 · Cited by 17 · see citing works
Rigg, J. L., & ZAFONTE, R. D. (2006) · Journal of Head Trauma Rehabilitation · doi:10.1097/00001199-200605000-00010 · Cited by 7 · see citing works
NFκB: cell survival or cell death?
Strozyk, E. A., & Kulms, D. (2005) · Signal Transduction · doi:10.1002/sita.200500070 · Cited by 6 · see citing works
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Sirtuin 1 alleviates neuroinflammation‐induced apoptosis after traumatic brain injury
Guan, W., Wang, J., Wu, Y., Zheng, X., Zeng, Y., Li, Y., & Chen, X. (2021) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.16534 · Cited by 26 · see citing works
Taylor, M. A., & Kokiko‐Cochran, O. N. (2024) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2024.1351685 · Cited by 15 · see citing works
Prvulović, M., Milanović, D., Vujovic, P. Z., Jović, M., Kanazir, S., Todorovic, S. T., & Mladenović, A. (2021) · The Journals of Gerontology Series A · doi:10.1093/gerona/glab353 · Cited by 14 · see citing works
Self-citationSerum/glucocorticoid regulated kinase 1 (SGK1) in neurological disorders: pain or gain
Howard, P. G., Zou, P., Zhang, Y., Huang, F., Tesic, V., Wu, C. Y.-C., & Lee, R. H.-C. (2024) · Experimental Neurology · doi:10.1016/j.expneurol.2024.114973 · Cited by 13 · see citing works
Suo, L., & Wang, M. (2022) · BMC Anesthesiology · doi:10.1186/s12871-022-01661-1 · Cited by 11 · see citing works
Potrebić, M., Pavković, Ž., Srbovan, M., Đmura, G., & Pešić, V. (2022) · Journal of the American Association for Laboratory Animal Science · doi:10.30802/aalas-jaalas-22-000032 · Cited by 6 · see citing works
Salem, M., Shaheen, M., & Borjac, J. M. (2022) · PharmaNutrition · doi:10.1016/j.phanu.2022.100308 · Cited by 6 · see citing works
Milosevic, A., Milosevic, K., Zivkovic, A., Lavrnja, I. C., Savić, D. Z., Bjelobaba, I. M., & Janjic, M. M. (2024) · Biomolecules · doi:10.3390/biom14081020 · Cited by 3 · see citing works
Ciric, J., Tešić, V., Milovanovic, N., Macura, I. J., Ivković, S., Kanazir, S., & Perović, M. (2022) · Brain Sciences · doi:10.3390/brainsci12101297
Self-citationTian, J., Wang, Y., Zhao, J., Guo, Z., Jiang, L., Zhou, L., Yin, Y., Ding, S., Wang, L., Lu, Y., et al. (2026) · Molecular Neurobiology · doi:10.1007/s12035-026-06112-3
- Authors
- Perović, M., Jović, M., Todorović, S., Đorđević, A. M., Milanović, D., Kanazir, S., & Lončarević-Vasiljković, N.
- Affiliations
- University of Belgrade (RS)
- Department of Neurobiology, Institute for Biological Research ‘Siniša Stanković’ – National Institute of Republic of Serbia, University of Belgrade Belgrade, Serbia
- ORCID
- Venue
- Nutritional Neuroscience
- Publisher
- Informa UK Limited
- Published
- 2020-06-01
- Volume
- 25
- Issue
- 3
- Pages
- 537-549
- ISSN
- 1028-415X (ISSN-L)
- 1476-8305
- OpenAlex
- W3029369270
- Cited by
- 10
- References
- 36
- Citations by year
- 2026: 1 · 2024: 3 · 2022: 4 · 2021: 2
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nutritional Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Digital Repository of Archived Publications (University of Belgrade, Institute for Biological Research Sinisa Stankovic) (submittedVersion)
- Funders
- Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja (ON173056)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Automotive and Human Injury Biomechanics
- Keywords
- dietary restriction, traumatic brain injury, neuroprotection, glucocorticoid receptor signaling, glucocorticoid receptor phosphorylation, 11β-hydroxysteroid dehydrogenase type 1, NF-κB p65, HSP70, anti-inflammatory activity, microglial activation, TNF-α, neuronal cell death, caspase-3, neuroplasticity
- MeSH terms
- Brain Injuries, Traumatic, Animals, Disease Models, Animal, Glucocorticoids, Humans, Middle Aged, NF-kappa B, Rats, Sprague-Dawley, Neuroprotective Agents, Rats
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