Biological evaluation of synthesized allicin and its transformation products obtained by microwaves in methanol: Antioxidant activity and effect on cell growth
Open accessAbstract
Allicin is the most biologically active substance present in garlic. It can be synthesized or obtained by extraction of fresh garlic. Transformation products of allicin are also biologically active. The aim of this study was to examine the antioxidant activity of synthesized allicin and its transformation products obtained using microwaves in methanol at 55 °C as well as their effect on HeLa cells growth. The antioxidant activity was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl radical) test. The effect on HeLa cells growth was determined by MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) test. For MTT test, allicin and its transformation products were dispersed in carmellose sodium solution and examined in concentrations ranging from 0.3 μg/mL to 3 mg/mL. Allicin showed stronger antioxidant activity than the transformation products. A maximum degree of neutralization of DPPH radicals, about 90%, was reached when the concentration of allicin was 2 mg/mL, with an EC50 (concentration of sample which is required for reduction of the initial concentration DPPH radicals to 50%) value of 0.37 mg/mL. In our study, allicin and its transformation products were not cytotoxic to HeLa cells under the examined conditions. The highest concentration of allicin and its transformation products had a slight antiproliferative effect, with a more pronounced effect of allicin, which reflected on the morphology of HeLa cells. The examined substances are safe to use on epithelial cells at concentrations up to 3 mg/mL when applied in carmellose sodium solution. Using carmellose sodium as a dispersing agent could be recommended as a good approach for testing liposoluble substances in liquid cell cultures.
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Allicin is a bright yellow oily liquid with a distinctive garlic odor and is very unstable, easily decomposed even at room temperature.
“Allicin, a bright yellow oily liquid that possesses a distinctive garlic odor and it is very unstable, therefore it can be easily decomposed even at room temperature [42].”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [42]
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Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review
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- Authors
- Ilić, D. P., Stojanović, S., Najman, S., Nikolić, V. D., Stanojević, L. P., Tačić, A., & Nikolić, L. B.
- Affiliations
- University of Nis (RS)
- Corresponding author
- Najman, Stevo
- Venue
- Biotechnology & Biotechnological Equipment
- Publisher
- Informa UK Limited
- Published
- 2015-01-02
- Volume
- 29
- Issue
- 1
- Pages
- 189-194
- ISSN
- 1310-2818 (ISSN-L)
- 1314-3530
- PMC
- PMC4433831
- OpenAlex
- W2090603968
- Cited by
- 20
- References
- 19
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 2 · 2017: 2
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Biotechnology & Biotechnological Equipment (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja (TR34012, III 41017, 41017, 34012)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
- Physical Sciences › Chemistry › Organic Chemistry › Chemical synthesis and alkaloids
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Keywords
- allicin, garlic, microwave-assisted synthesis, antioxidant activity, DPPH radical scavenging, HeLa cells, cytotoxicity, antiproliferative activity, cell culture media
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