Long noncoding RNA MEG3 is downregulated in cervical cancer and affects cell proliferation and apoptosis by regulating miR-21
Open accessAbstract
Recent research has found that long noncoding RNAs (lncRNAs) were involved in various human cancers. However, the role of these lncRNAs in cervical cancer remains unexplored. Therefore, we aimed to investigate the biological function of maternally expressed gene 3 (MEG3), a cancer-related lncRNA, and its underlying mechanism in cervical cancer. In this study, MEG3 expression of 108 patients' cervical cancer tissues and adjacent normal tissues was detected by quantitative real-time PCR analysis (qRT-PCR), and the functional effect of MEG3 was determined in vitro assays. We observed that MEG3 was downregulated in cervical cancer tissues, compared to the adjacent normal tissues, and was negatively related with FIGO stages, tumor size, lymphatic metastasis, HR-HPV infection and the expression of homo sapiens microRNA-21 (miR-21). Furthermore, we focused on the function and molecular mechanism of MEG3, finding that overexpression of MEG3 reduced the level of miR-21-5p expression, causing inhibition of proliferation and increased apoptosis in cervical cancer cells. In summary, our findings indicate that MEG3 function as a tumor suppressor by regulating miR-21-5p, resulting in the inhibition of tumor growth in cervical cancer. As a result, this study improves our understanding of the function of MEG3 in cervical cancer and will help to provide new potential target sites for cervical cancer treatment.
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MEG3 serves as a ceRNA for miR-21 to regulate cell proliferation or apoptosis in some tumors.
“More significantly, studies have revealed that MEG3 serves as a ceRNA for miR-21 to regulate cell proliferation or apoptosisin in some tumors34,35, but whether MEG3 competes with mRNA for miR-21 in ischemia and contributes to ischemic neuronal death remained unexplored.”
From Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate ischemic neuronal death by targeting miR-21/PDCD4 signaling pathway (2017) · cites this paper as [34]
The Results and Discussion (page not provided) report that MEG3 regulates miR-21-5p and affects proliferation and apoptosis in cervical cancer cells. They do not establish that MEG3 acts as a competing endogenous RNA (ceRNA) for miR-21; the Discussion says the exact mechanism of their interaction requires further study. (LLM, conf=0.88)
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Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals
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- Authors
- Zhang, J., Yao, T., Wang, Y., Yu, J., Liu, Y., & Lin, Z.
- Affiliations
- Sun Yat-sen University (CN)
- Corresponding author
- Lin, Zhongqiu
- Venue
- Cancer Biology & Therapy
- Publisher
- Informa UK Limited
- Published
- 2015-11-17
- Volume
- 17
- Issue
- 1
- Pages
- 104-113
- ISSN
- 1538-4047 (ISSN-L)
- 1555-8576
- PMC
- PMC4847830
- OpenAlex
- W2174891444
- Cited by
- 305
- References
- 3
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 14 · 2023: 27 · 2022: 18 · 2021: 30 · 2020: 49 · 2019: 49 · 2018: 50 · 2017: 43 · 2016: 8 · 2015: 1
- FWCI
- 11.24
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Cancer Biology & Therapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81572575)
- Natural Science Foundation of Guangdong Province (2014A030313109)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Circular RNAs in diseases
- Keywords
- MEG3, long non-coding RNA, cervical cancer, miR-21-5p, tumor suppressor, cell proliferation, apoptosis, microRNA regulation, HPV infection, lymph node metastasis, FIGO stage
- MeSH terms
- Aged, Uterine Cervical Neoplasms, Female, Humans, Lymphatic Metastasis, Middle Aged, Gene Expression Regulation, Neoplastic, Apoptosis, DNA Methylation, MicroRNAs, Cell Line, Tumor, Cell Proliferation, RNA, Long Noncoding
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