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Cited by 131Pluripotent Stem Cells ResearchNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Large-scale generation of human iPSC-derived neural stem cells/early neural progenitor cells and their neuronal differentiation

Open access
D’Aiuto, Leonardo, Zhi, Yun, Kumar Das, Dhanjit, Wilcox, Madeleine R, Johnson, Jon W, McClain, Lora, MacDonald, Matthew L, Di Maio, Roberto, Schurdak, Mark E, Piazza, Paolo, Viggiano, Luigi, Sweet, Robert, Kinchington, Paul R, Bhattacharjee, Ayantika G, Yolken, Robert, Nimgaonka, Vishwajit L·2014·Organogenesis
DOI 10.1080/15476278.2015.1011921PMC PMC4594592
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Abstract

Induced pluripotent stem cell (iPSC)-based technologies offer an unprecedented opportunity to perform high-throughput screening of novel drugs for neurological and neurodegenerative diseases. Such screenings require a robust and scalable method for generating large numbers of mature, differentiated neuronal cells. Currently available methods based on differentiation of embryoid bodies (EBs) or directed differentiation of adherent culture systems are either expensive or are not scalable. We developed a protocol for large-scale generation of neuronal stem cells (NSCs)/early neural progenitor cells (eNPCs) and their differentiation into neurons. Our scalable protocol allows robust and cost-effective generation of NSCs/eNPCs from iPSCs. Following culture in neurobasal medium supplemented with B27 and BDNF, NSCs/eNPCs differentiate predominantly into vesicular glutamate transporter 1 (VGLUT1) positive neurons. Targeted mass spectrometry analysis demonstrates that iPSC-derived neurons express ligand-gated channels and other synaptic proteins and whole-cell patch-clamp experiments indicate that these channels are functional. The robust and cost-effective differentiation protocol described here for large-scale generation of NSCs/eNPCs and their differentiation into neurons paves the way for automated high-throughput screening of drugs for neurological and neurodegenerative diseases.

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Crossref · 10.1080/15476278.2015.1011921

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Research works printed but not linked: 2

  1. Kirkeby A, 2012, Front Cell Neurosci, 6, 64
  2. Gonzalez-Perez O, 2012, Biol Biomed Rep, 2, 59

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Metadata

Authors
D’Aiuto, L., Zhi, Y., Kumar Das, D., Wilcox, M. R., Johnson, J. W., McClain, L., MacDonald, M. L., Di Maio, R., Schurdak, M. E., Piazza, P., Viggiano, L., Sweet, R., Kinchington, P. R., Bhattacharjee, A. G., Yolken, R., & Nimgaonka, V. L.
Affiliations
  • University of Pittsburgh (US)
  • University of Bari Aldo Moro (IT)
  • Johns Hopkins University (US)
Corresponding author
Nimgaonka, Vishwajit L
ORCID
Venue
Organogenesis
Publisher
Informa UK Limited
Published
2014-10-02
Volume
10
Issue
4
Pages
365-377
ISSN
  • 1547-6278 (ISSN-L)
  • 1555-8592
DOI
10.1080/15476278.2015.1011921
PMC
PMC4594592
OpenAlex
W1817943252
Cited by
131
References
33
Citations by year
2026: 3 · 2025: 9 · 2024: 13 · 2023: 6 · 2022: 15 · 2021: 22 · 2020: 17 · 2019: 17 · 2018: 11 · 2017: 11 · 2016: 5 · 2015: 2
FWCI
2.46
Type
journal article
Language
en
Update notices
correction (2015-08-07)
Open access
Locations
  • Organogenesis (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
Funders
  • U.S. Department of Veterans Affairs
  • Indo-US Science and Technology Forum
  • National Science Council
  • China Scholarship Council
  • National Institutes of Health (MH071533, MH045817, MH63480)
  • Cancer Institute, University of Pittsburgh (P30CA047904)
  • National Institute of Mental Health (MH071533, R01MH042191, 07R-1712, (MH 63480, R01MH071533, R01MH063480, MH045817, R01MH045817, T32MH016804)
  • National Cancer Institute (P30CA047904)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
induced pluripotent stem cells, neural stem cells, neuronal differentiation, VGLUT1, ligand-gated ion channels, synaptic proteins, high-throughput drug screening, neurodegenerative diseases, iPSC-derived neurons
MeSH terms
Cell Differentiation, Cells, Cultured, Humans, Neurons, Cell Proliferation, Induced Pluripotent Stem Cells, Neural Stem Cells, Batch Cell Culture Techniques

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