Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver
Open accessAbstract
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements of Porcine DLs could be achieved using AgNPs.
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Silver nanoparticle-treated scaffolds exhibited reduced host inflammatory reactions and higher M2 macrophage polarization upon subcutaneous implantation in mice compared to glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide-treated scaffolds.
“Furthermore, silver nanoparticle-treated scaffolds exhibited reduced host inflammatory reactions and higher M2 macrophage polarization upon subcutaneous implantation in mice compared to glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide-treated scaffolds [177].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [177]
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Wang, Y., Bao, J., Wu, X., Wu, Q., Li, Y., Zhou, Y., Li, L., & Bu, H. (2016) · Scientific Reports · doi:10.1038/srep24779 · Cited by 101 · see citing works
Mechanostructure and composition of highly reproducible decellularized liver matrices
Mattei, G., Di Patria, V., Tirella, A., Alaimo, A., Elia, G., Corti, A., Paolicchi, A., & Ahluwalia, A. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.10.023 · Cited by 78 · see citing works
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Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“Silver nanoparticle have been employed for cross-linking of porcine liver slices, preserving ultrastructure, enhancing resistance against in vitro degradation, and demonstrating good cyto-compatibility with hepatocellular carcinoma cell line (HepG2 cells) and EA. hy926 cells [177].”
BackgroundSelf-citationAhmed, E., Saleh, T., Yu, L., Song, S., Park, K., Kwak, H., & Woo, H. (2020) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36988 · Cited by 23 · see citing works
Self-citationStrategies for decellularization, re-cellularization and crosslinking in liver bioengineering
Wang, J., & Jin, X. (2024) · The International Journal of Artificial Organs · doi:10.1177/03913988231218566 · Cited by 3 · see citing works
- Authors
- Saleh, T., Ahmed, E., Yu, L., Hussein, K., Park, K.-M., Lee, Y.-S., Kang, B.-J., Choi, K.-Y., Choi, S., Kang, K.-S., & Woo, H.-M.
- Affiliations
- Kangwon National University (KR)
- Assiut University (EG)
- Chungbuk National University (KR)
- Seoul National University (KR)
- Corresponding author
- Woo, Heung-Myong
- ORCID
- Venue
- Artificial Cells, Nanomedicine, and Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2018-03-27
- Volume
- 46
- Issue
- sup2
- Pages
- 273-284
- ISSN
- 2169-1401 (ISSN-L)
- 2169-141X
- OpenAlex
- W2795133409
- Cited by
- 41
- References
- 2
- Citations by year
- 2025: 7 · 2024: 4 · 2023: 6 · 2022: 6 · 2021: 5 · 2020: 7 · 2019: 3 · 2018: 3
- FWCI
- 4.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Artificial Cells Nanomedicine and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Rural Development Administration, Republic of Korea
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- silver nanoparticles, porcine liver, crosslinking agents, structural stability, biocompatibility, collagen fibers, porosity, in vitro degradation, cytocompatibility, HepG2 cells, glutaraldehyde, N-hydroxysuccinimide, M2 macrophage polarization, tissue engineering scaffolds
- MeSH terms
- Animals, Dose-Response Relationship, Drug, Humans, Liver, Materials Testing, Silver, Swine, Collagenases, Metal Nanoparticles, Hep G2 Cells
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