Mechanostructure and composition of highly reproducible decellularized liver matrices
Open accessAbstract
Despite the increasing number of papers on decellularized scaffolds, there is little consensus on the optimum method of decellularizing biological tissue such that the micro-architecture and protein content of the matrix are conserved as far as possible. Focusing on the liver, the aim of this study was therefore to develop a method for the production of well-characterized and reproducible matrices that best preserves the structure and composition of the native extra cellular matrix (ECM). Given the importance of matrix stiffness in regulating cell response, the mechanical properties of the decellularized tissue were also considered. The testing and analysis framework is based on the characterization of decellularized and untreated samples in the same reproducible initial state (i.e., the equilibrium swollen state). Decellularized ECM (dECM) were characterized using biochemical, histological, mechanical and structural analyses to identify the best procedure to ensure complete cell removal while preserving most of the native ECM structure and composition. Using this method, sterile decellularized porcine ECM with highly conserved intra-lobular micro-structure and protein content were obtained in a consistent and reproducible manner using the equilibrium swollen state of tissue or matrix as a reference. A significant reduction in the compressive elastic modulus was observed for liver dECM with respect to native tissue, suggesting a re-examination of design parameters for ECM-mimicking scaffolds for engineering tissues in vitro.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The toxic effect of H2O2 gas plasma may be attributed to the generation of free radicals after H2O2 treatment, which negatively affects the matrix.
“This may be attributed to the generation of free radicals after H2O2 treatment, which negatively affects the matrix.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [184]
Chloroform vapor and immersion in PAA showed no cytotoxic effect on hepatocytes, whereas H2O2 gas plasma treatment (with or without chloroform) showed a very toxic effect on hepatocytes.
“Chloroform vapor and immersion in PAA showed no cytotoxic effect on the hepatocytes, whereas H2O2 gas plasma treatment (with or without chloroform) showed a very toxic effect on hepatocytes.”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [184]
Cites
Decellularization of tissues and organs
Gilbert, T. W., Sellaro, T. L., & Badylak, S. F. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.014 · Cited by 2,222 · see citing works
Uygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., et al. (2010) · Nature Medicine · doi:10.1038/nm.2170 · Cited by 1,370 · see citing works
Regeneration and orthotopic transplantation of a bioartificial lung
Ott, H. C., Clippinger, B., Conrad, C., Schuetz, C., Pomerantseva, I., Ikonomou, L., Kotton, D., & Vacanti, J. P. (2010) · Nature Medicine · doi:10.1038/nm.2193 · Cited by 1,090 · see citing works
Badylak, S. F., Taylor, D., & Uygun, K. (2011) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124743 · Cited by 1,041 · see citing works
Intrinsic extracellular matrix properties regulate stem cell differentiation
Reilly, G. C., & Engler, A. J. (2009) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2009.09.009 · Cited by 782 · see citing works
The use of whole organ decellularization for the generation of a vascularized liver organoid
Baptista, P. M., Siddiqui, M. M., Lozier, G., Rodriguez, S. R., Atala, A., & Soker, S. (2011) · Hepatology · doi:10.1002/hep.24067 · Cited by 681 · see citing works
Reing, J. E., Brown, B. N., Daly, K. A., Freund, J. M., Gilbert, T. W., Hsiong, S. X., Huber, A., Kullas, K. E., Tottey, S., Wolf, M. T., et al. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.07.083 · Cited by 475 · see citing works
Embryonic Stem Cells Proliferate and Differentiate when Seeded into Kidney Scaffolds
Ross, E. A., Williams, M. J., Hamazaki, T., Terada, N., Clapp, W. L., Adin, C. A., Ellison, G. W., Jorgensen, M. J., & Batich, C. D. (2009) · Journal of the American Society of Nephrology · doi:10.1681/asn.2008111196 · Cited by 380 · see citing works
Woods, T., & Gratzer, P. F. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.05.066 · Cited by 375 · see citing works
Nelson, C. M., & Bissell, M. J. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.05.001 · Cited by 332 · see citing works
Cebotari, S., Tudorache, I., Jaekel, T., Hilfiker, A., Dorfman, S. E., Ternes, W., Haverich, A., & Lichtenberg, A. (2010) · Artificial Organs · doi:10.1111/j.1525-1594.2009.00796.x · Cited by 272 · see citing works
Sohlenius-Sternbeck, A.-K. (2006) · Toxicology in Vitro · doi:10.1016/j.tiv.2006.06.003 · Cited by 261 · see citing works
Wang, Y., Cui, C.-B., Yamauchi, M., Miguez, P., Roach, M., Malavarca, R., Costello, J. M., Cardinale, V., Wauthier, E., Barbier, C., et al. (2011) · Hepatology · doi:10.1002/hep.24012 · Cited by 220 · see citing works
Bolland, F., Korossis, S., Wilshaw, S., Ingham, E., Fisher, J., Kearney, J. N., & Southgate, J. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.10.005 · Cited by 191 · see citing works
Development of acellular dermis from porcine skin using periodic pressurized technique
Prasertsung, I., Kanokpanont, S., Bunaprasert, T., Thanakit, V., & Damrongsakkul, S. (2007) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.30938 · Cited by 145 · see citing works
Hydrated xenogeneic decellularized tracheal matrix as a scaffold for tracheal reconstruction
Remlinger, N. T., Czajka, C. A., Juhas, M. E., Vorp, D. A., Stolz, D. B., Badylak, S. F., Gilbert, S., & Gilbert, T. W. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.01.067 · Cited by 140 · see citing works
Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix
Lang, R., Stern, M. M., Smith, L., Liu, Y., Bharadwaj, S., Liu, G., Baptista, P. M., Bergman, C. R., Söker, S., Yoo, J. J., et al. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.06.005 · Cited by 136 · see citing works
TRITON‐X is most effective among three decellularization agents for ACL tissue engineering
Vavken, P., Joshi, S. M., & Murray, M. M. (2009) · Journal of Orthopaedic Research® · doi:10.1002/jor.20932 · Cited by 134 · see citing works
Modular extracellular matrices: Solutions for the puzzle
Serban, M. A., & Prestwich, G. D. (2008) · Methods · doi:10.1016/j.ymeth.2008.01.010 · Cited by 108 · see citing works
Image-guided, Lobe-specific Hydrodynamic Gene Delivery to Swine Liver
Kamimura, K., Suda, T., Xu, W., Zhang, G., & Liu, D. (2009) · Molecular Therapy · doi:10.1038/mt.2008.294 · Cited by 99 · see citing works
A low shear stress modular bioreactor for connected cell culture under high flow rates
Mazzei, D., Guzzardi, M. A., Giusti, S., & Ahluwalia, A. D. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22671 · Cited by 97 · see citing works
Self-citationPreparation of Ex Vivo –Based Biomaterials Using Convective Flow Decellularization
Montoya, C. V., & McFetridge, P. S. (2009) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2008.0372 · Cited by 93 · see citing works
Scale-dependent mechanical properties of native and decellularized liver tissue
Evans, D. W., Moran, E. C., Baptista, P. M., Söker, S., & Sparks, J. L. (2012) · Biomechanics and Modeling in Mechanobiology · doi:10.1007/s10237-012-0426-3 · Cited by 90 · see citing works
Marchesseau, S., Heimann, T., Chatelin, S., Willinger, R., & Delingette, H. (2010) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2010.09.005 · Cited by 86 · see citing works
Mechanical Characterization of Porcine Abdominal Organs
TAMURA, A., Omori, K., Kazuo, M. I. K. I., Lee, J. B., Yang, K. H., & King, A. I. (2002) · SAE technical papers on CD-ROM/SAE technical paper series · doi:10.4271/2002-22-0003 · Cited by 80 · see citing works
Wiegand, C., Abel, M., Ruth, P., Wilhelms, T., Schulze, D., Norgauer, J., & Hipler, U. (2009) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.31338 · Cited by 69 · see citing works
Burch, P. T., Kaza, A. K., Lambert, L. M., Holubkov, R., Shaddy, R. E., & Hawkins, J. A. (2010) · The Annals of Thoracic Surgery · doi:10.1016/j.athoracsur.2010.05.024 · Cited by 66 · see citing works
Remodeling of ACL Allografts is Inhibited by Peracetic Acid Sterilization
Scheffler, S. U., Gonnermann, J., Kamp, J., Przybilla, D., & Prüß, A. (2008) · Clinical Orthopaedics and Related Research · doi:10.1007/s11999-008-0288-2 · Cited by 52 · see citing works
General Techniques of Cell Culture
Harrison, M. A., & Rae, I. F. (1997) · Cambridge University Press eBooks · doi:10.1017/cbo9780511623226 · Cited by 41 · see citing works
Lee, L. T., Deas, J. E., & Howe, C. (1978) · Journal of Immunological Methods · doi:10.1016/0022-1759(78)90009-1 · Cited by 23 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Bioactivity of Cartilage Extracellular Matrix in Articular Cartilage Regeneration
Sutherland, A. J., Converse, G. L., Hopkins, R. A., & Detamore, M. S. (2014) · Advanced Healthcare Materials · doi:10.1002/adhm.201400165 · Cited by 180 · see citing works
Perepelyuk, M., Chin, L., Cao, X., van Oosten, A., Shenoy, V. B., Janmey, P. A., & Wells, R. G. (2016) · PLoS ONE · doi:10.1371/journal.pone.0146588 · Cited by 129 · see citing works
Kawecki, M., Łabuś, W., Klama‐Baryła, A., Kitala, D., Kraut, M., Glik, J., Misiuga, M., Nowak, M., Bielecki, T., & Kasperczyk, A. (2017) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33865 · Cited by 129 · see citing works
Tao, M., Ao, T., Mao, X., Yan, X., Javed, R., Hou, W., Wang, Y., Sun, C., Lin, S., Yu, T., et al. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.02.010 · Cited by 119 · see citing works
Park, K.-M., Hussein, K. H., Hong, S.-H., Ahn, C., Yang, S.-R., Park, S.-M., Kweon, O.-K., Kim, B.-M., & Woo, H.-M. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2015.0313 · Cited by 113 · see citing works
Hussein, K. H., Park, K.-M., Kang, K.-S., & Woo, H.-M. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.04.042 · Cited by 100 · see citing works
Verstegen, M. M. A., Willemse, J., van den Hoek, S., Kremers, G., Luider, T. M., van Huizen, N. A., Willemssen, F. E. J. A., Metselaar, H. J., IJzermans, J. N. M., van der Laan, L. J. W., et al. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0095 · Cited by 94 · see citing works
Das, S., Jegadeesan, J. T., & Basu, B. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.3c01271 · Cited by 102 · see citing works
Devalliere, J., Chen, Y., Dooley, K., Yarmush, M. L., & Uygun, B. E. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.07.046 · Cited by 84 · see citing works
Sample, testing and analysis variables affecting liver mechanical properties: A review
Mattei, G., & Ahluwalia, A. D. (2016) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.08.055 · Cited by 83 · see citing works
Self-citationMassaro, M. S., Pálek, R., Rosendorf, J., Červenková, L., Liška, V., & Moulisová, V. (2021) · Materials Science and Engineering: C · doi:10.1016/j.msec.2021.112203 · Cited by 82 · see citing works
Decellularized scaffolds for tissue engineering: Current status and future perspective
Rajab, T. K., O’Malley, T. J., & Tchantchaleishvili, V. (2020) · Artificial Organs · doi:10.1111/aor.13701 · Cited by 79 · see citing works
Developing tissue engineering strategies for liver regeneration
Wang, J., Huang, D., Yu, H., Cheng, Y., Ren, H., & Zhao, Y. (2022) · Engineered Regeneration · doi:10.1016/j.engreg.2022.02.003 · Cited by 81 · see citing works
Recent advances in liver engineering with decellularized scaffold
Dai, Q., Jiang, W., Huang, F., Song, F., Zhang, J., & Zhao, H. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.831477 · Cited by 57 · see citing works
Mattei, G., Ferretti, C., Tirella, A., Ahluwalia, A. D., & Mattioli‐Belmonte, M. (2015) · Scientific Reports · doi:10.1038/srep10778 · Cited by 52 · see citing works
Self-citationMattei, G., Magliaro, C., Pirone, A., & Ahluwalia, A. D. (2017) · Artificial Organs · doi:10.1111/aor.12925 · Cited by 50 · see citing works
Self-citationViscoelastic characterisation of pig liver in unconfined compression
Mattei, G., Tirella, A., Gallone, G., & Ahluwalia, A. D. (2014) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2014.05.017 · Cited by 47 · see citing works
Self-citationErgun, C., Parmaksiz, M., Vurat, M. T., Elçin, A. E., & Elçin, Y. M. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.12.086 · Cited by 45 · see citing works
Sun, D., Liu, Y., Wang, H., Deng, F., Zhang, Y., Zhao, S., Ma, X., Wu, H., & Sun, G. (2018) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.11.103 · Cited by 45 · see citing works
Milan, P. B., Masoumi, F., Biazar, E., Jalise, S. Z., & Mehrabi, A. (2024) · Macromolecular Bioscience · doi:10.1002/mabi.202400322 · Cited by 41 · see citing works
Saleh, T., Ahmed, E., Yu, L., Hussein, K., Park, K.-M., Lee, Y.-S., Kang, B.-J., Choi, K.-Y., Choi, S., Kang, K.-S., et al. (2018) · Artificial Cells, Nanomedicine, and Biotechnology · doi:10.1080/21691401.2018.1457037 · Cited by 41 · see citing works
Predeina, A. L., Сергеевна, Д. М., & Vinogradov, V. V. (2020) · Journal of Materials Chemistry B · doi:10.1039/d0tb01751e · Cited by 39 · see citing works
On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids
Mattei, G., Magliaro, C., Giusti, S., Ramachandran, S. D., Heinz, S., Braspenning, J., & Ahluwalia, A. D. (2017) · PLoS ONE · doi:10.1371/journal.pone.0173206 · Cited by 38 · see citing works
Self-citationRijns, L., Rutten, M. G. T. A., Vrehen, A. F., Aldana, A. A., Baker, M. B., & Dankers, P. Y. W. (2024) · Nanoscale · doi:10.1039/d4nr02088j · Cited by 37 · see citing works
Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts
Wang, Y., Nicolas, C. T., Chen, H. S., Ross, J. J., Lorenzo, S. B. D., & Nyberg, S. L. (2016) · Cells Tissues Organs · doi:10.1159/000452761 · Cited by 36 · see citing works
Poornejad, N., Nielsen, J. J., Morris, R. J., Gassman, J. R., Reynolds, P. R., Roeder, B. L., & Cook, A. D. (2015) · Journal of Biomaterials Applications · doi:10.1177/0885328215615760 · Cited by 35 · see citing works
Gelatin-Based Hybrid Hydrogels as Matrices for Organoid Culture
Carpentier, N., Ye, S., Delemarre, M. D., der Meeren, L. V., Skirtach, A. G., van der Laan, L. J. W., Schneeberger, K., Spee, B., Dubruel, P., & Vlierberghe, S. V. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.2c01496 · Cited by 34 · see citing works
Recent Advances in Decellularization and Recellularization for Tissue-Engineered Liver Grafts
Wang, Y., Nicolas, C. T., Chen, H. S., Ross, J. J., Lorenzo, S. B. D., & Nyberg, S. L. (2017) · Cells Tissues Organs · doi:10.1159/000479597 · Cited by 33 · see citing works
Sui, J., Pragnère, S., & Kurniawan, N. A. (2025) · Biomaterials Science · doi:10.1039/d5bm00210a · Cited by 32 · see citing works
Yang, W., Chen, Q., Xia, R., Zhang, Y., Shuai, L., Lai, J., You, X., Jiang, Y., Bie, P., Zhang, L., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.05.026 · Cited by 30 · see citing works
Hydrogel-Based Strategies for Liver Tissue Engineering
Zhang, Y., Li, L., Dong, L., Cheng, Y., Huang, X., Xue, B., Jiang, C., Cao, Y., & Yang, J. (2024) · Chem & Bio Engineering · doi:10.1021/cbe.4c00079 · Cited by 31 · see citing works
In vitro assessment of electrospun polyamide‐6 scaffolds for esophageal tissue engineering
Zhuravleva, M. N., Gilazieva, Z., Grigoriev, T. Е., Shepelev, A. D., Tenchurin, T. K., Kamyshinsky, R. A., Krasheninnikov, S. V., Орлов, С. В., Caralogli, G., Archipova, S., et al. (2018) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34116 · Cited by 28 · see citing works
Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“Mattei et al. sterilized freeze-dried decellularized porcine liver discs by exposing them to chloroform vapor, H2O2 gas plasma, a combination of chloroform vapor and H2O2 gas plasma, or PAA, before performing direct contact cytotoxicity assay with hepatocytes for up to 48 h [184].”
MethodEverwien, H., de Schellenberger, A. A., Haep, N., Tzschätzsch, H., Pratschke, J., Sauer, I. M., Braun, J., Hillebrandt, K. H., & Sack, I. (2020) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2020.103640 · Cited by 22 · see citing works
Asadi, M., Khalili, M., Lotfi, H., Moghaddam, S. V., Zarghami, N., André, H., & Alizadeh, E. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119373 · Cited by 22 · see citing works
Natural extracellular matrix scaffolds recycled from human salivary digests: a morphometric study
Lilliu, M., Seo, Y., Isola, M., Charbonneau, A., Zeitouni, A. G., El‐Hakim, M., & Tran, S. (2016) · Oral Diseases · doi:10.1111/odi.12444 · Cited by 21 · see citing works
Zhu, X., Wu, Q., He, Y., Gao, M., Li, Y., Peng, W., Li, S., Liu, Y., Zhang, R., & Bao, J. (2022) · ACS Omega · doi:10.1021/acsomega.1c06302 · Cited by 20 · see citing works
Zheng, X., Xiang, J., Wu, W., Wang, B., Liu, W., Gao, R., Dong, D., & Lv, Y. (2015) · Biomedical Materials · doi:10.1088/1748-6041/10/4/045023 · Cited by 20 · see citing works
Accounting for Material Changes in Decellularized Tissue with Underutilized Methodologies
Hansen, R. A. B., Wang, X., Kaw, G., Pierre, V., & Senyo, S. E. (2021) · BioMed Research International · doi:10.1155/2021/6696295 · Cited by 18 · see citing works
Gelatin-Based Hybrid Hydrogel Scaffolds: Toward Physicochemical Liver Mimicry
Carpentier, N., der Meeren, L. V., Skirtach, A. G., Devisscher, L., Vlierberghe, H. V., Dubruel, P., & Vlierberghe, S. V. (2022) · Biomacromolecules · doi:10.1021/acs.biomac.2c00643 · Cited by 17 · see citing works
Mußbach, F., Settmacher, U., Dirsch, O., Xie, C., & Dahmen, U. (2016) · European Surgical Research · doi:10.1159/000446211 · Cited by 18 · see citing works
Bioinspired liver scaffold design criteria
Mattei, G., Magliaro, C., Pirone, A., & Ahluwalia, A. D. (2018) · Organogenesis · doi:10.1080/15476278.2018.1505137 · Cited by 18 · see citing works
Self-citationSaleh, T., Ahmed, E., Yu, L., Kwak, H., Kang, B., Park, K., Choi, K., Kim, B., Kang, K., & Woo, H. (2019) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2019.04.017 · Cited by 16 · see citing works
Meekel, J. P., Mattei, G., Costache, V. S., Balm, R., Blankensteijn, J. D., & Yeung, K. K. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.07.019 · Cited by 15 · see citing works
Self-citationRecent advances in optimization of liver decellularization procedures used for liver regeneration
Khajavi, M., Hashemi, M., & Kalalinia, F. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119801 · Cited by 14 · see citing works
Flexor Tendon Sheath Engineering Using Decellularized Porcine Pericardium
Megerle, K., Woon, C. Y.-L., Kraus, A., Raghavan, S. S., Pham, H. M., & Chang, J. (2016) · Plastic & Reconstructive Surgery · doi:10.1097/prs.0000000000002459 · Cited by 14 · see citing works
Bioprinting of hepatic tissue model using photocrosslinkable dECM-containing composite hydrogel
Tabatabaei Rezaei, N., Kumar, H., Liu, H., Rajeev, A., Natale, G., Lee, S. S., Park, S. S., & Kim, K. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.101824 · Cited by 13 · see citing works
A Survival Model of In Vivo Partial Liver Lobe Decellularization Towards In Vivo Liver Engineering
Wang, A., Kuriata, O., Xu, F., Nietzsche, S., Gremse, F., Dirsch, O., Settmacher, U., & Dahmen, U. (2019) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2019.0194 · Cited by 13 · see citing works
Pospelov, A. D., Kutova, O. M., Efremov, Y. M., Nekrasova, A. A., Trushina, D. B., Gefter, S. D., Cherkasova, E. I., Timofeeva, L. B., Timashev, P. S., Zvyagin, A. V., et al. (2023) · Cells · doi:10.3390/cells12162030 · Cited by 12 · see citing works
Kim, W.-J., & Kim, G. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.02.001 · Cited by 11 · see citing works
Syndecan-4 promotes vascular beds formation in tissue engineered liver via thrombospondin 1
Hu, X. V., Chen, J., Huang, H., Yin, S., Zheng, S., & Zhou, L. (2020) · Bioengineered · doi:10.1080/21655979.2020.1846897 · Cited by 11 · see citing works
Harvesting decellularized liver extracellular matrix from rodents for 3D scaffold fabrication
Kasturi, M., & Vasanthan, K. S. (2024) · Artificial Cells Nanomedicine and Biotechnology · doi:10.1080/21691401.2024.2319893 · Cited by 10 · see citing works
HisTOOLogy: an open‐source tool for quantitative analysis of histological sections
Magliaro, C., Tirella, A., Mattei, G., Pirone, A., & Ahluwalia, A. D. (2015) · Journal of Microscopy · doi:10.1111/jmi.12292 · Cited by 10 · see citing works
Self-citationGupta, S., Sharma, A., Petrovski, G., & Verma, R. S. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1221159 · Cited by 8 · see citing works
New advances in liver decellularization and recellularization: innovative and critical technologies
Lin, Y.-Q., Wang, L., Wang, J., Pan, L., Zhu, G., Liu, W., Braddock, M., & Zheng, M. (2015) · Expert Review of Gastroenterology & Hepatology · doi:10.1586/17474124.2015.1058155 · Cited by 8 · see citing works
Whole-Liver Decellularization: Advances and Insights into Current Understanding
Fathi, I., & Eltawila, A. M. (2017) · InTech eBooks · doi:10.5772/intechopen.69487 · Cited by 7 · see citing works
Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome
Mattei, G., Cristiani, I., Magliaro, C., & Ahluwalia, A. D. (2015) · PeerJ · doi:10.7717/peerj.932 · Cited by 7 · see citing works
Self-citationEffect of ligation on the viscoelastic properties of liver tissues
Liu, D., Li, G., Su, C., Zheng, Y., Jiang, Y., Qian, L., & Cao, Y. (2018) · Journal of Biomechanics · doi:10.1016/j.jbiomech.2018.05.018 · Cited by 7 · see citing works
Bioengineering liver tissue by repopulation of decellularised scaffolds
Afzal, Z., & Huguet, E. (2023) · World Journal of Hepatology · doi:10.4254/wjh.v15.i2.151 · Cited by 6 · see citing works
Bobrova, M. M., Safonova, L. A., Агапова, О. И., Крашенинников, М. Е., Shagidulin, M. Y., & Agapov, I. I. (2015) · Современные технологии в медицине · doi:10.17691/stm2015.7.4.01 · Cited by 6 · see citing works
„Liver engineering“ als neue Quelle von Spenderorganen
Mußbach, F., Dahmen, U., Dirsch, O., & Settmacher, U. (2015) · Der Chirurg · doi:10.1007/s00104-015-0015-y · Cited by 5 · see citing works
Yu, L., Yang, J., Wang, L., Wang, W., & Yan, Y. (2018) · Journal of Medical and Biological Engineering · doi:10.1007/s40846-018-0438-2 · Cited by 5 · see citing works
Sparks, J. (2017) · doi:10.1002/9783527689934.ch9 · Cited by 2 · see citing works
Bobrova, M. M., Safonova, L. A., Efimov, А. Е., Iljinsky, I. M., Агапова, О. И., & Agapov, I. I. (2021) · Biomedical Materials · doi:10.1088/1748-605x/ac058b · Cited by 4 · see citing works
Strategies for decellularization, re-cellularization and crosslinking in liver bioengineering
Wang, J., & Jin, X. (2024) · The International Journal of Artificial Organs · doi:10.1177/03913988231218566 · Cited by 3 · see citing works
The Decellularization of Whole Organs
Huang, Y. H., Yue, H., Lian, Z., & Li, X. (2021) · Springer eBooks · doi:10.1007/978-981-33-6962-7_5 · Cited by 3 · see citing works
Wani, S. I., Mir, T. A., Nakamura, M. M., Tsuchiya, T., Alzhrani, A., Iwanaga, S., Arai, K., Alshehri, E. A., Shamma, T., Obeid, D. A., et al. (2024) · Bioprinting · doi:10.1016/j.bprint.2024.e00355 · Cited by 2 · see citing works
Extracellular matrix cues as drivers of liver zonation: A framework for in vitro biomaterial design
Criel, H., Grootaert, C., & Camp, J. V. (2025) · Acta Biomaterialia · doi:10.1016/j.actbio.2025.12.015 · Cited by 2 · see citing works
3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
Fan, W., Li, Y., Kunimoto, K., & Torok, N. J. (2022) · Journal of Visualized Experiments · doi:10.3791/63106 · Cited by 3 · see citing works
Dormancy in Metastatic Colorectal Cancer: Tissue Engineering Opportunities for In Vitro Modeling
VandenHeuvel, S. N., Nash, L. L., & Raghavan, S. A. (2025) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2025.0009 · Cited by 2 · see citing works
Cidonio, G., Brancato, V., Scognamiglio, C., Marzi, M., Barbetta, A., Riminucci, M., Nicassio, F., & Ruocco, G. (2023) · Tissue Engineering Part A · doi:10.1089/ten.tea.2023.29046.abstracts · Cited by 1 · see citing works
Bioengineering Liver Transplantation
Verstegen, M. M. A., Spee, B., & van der Laan, L. J. W. (2019) · Bioengineering · doi:10.3390/bioengineering6040096 · Cited by 1 · see citing works
Bioengineering Liver Transplantation
van der Laan, L. J. W., Spee, B., & Verstegen, M. M. A. (2019) · MDPI eBooks · doi:10.3390/books978-3-03921-745-8 · Cited by 1 · see citing works
Decellularized Cartilage as a Bioactive Biomaterial for Cartilage Tissue Engineering
Sutherland, A. J. (2014) · KU ScholarWorks (The University of Kansas)
„Liver engineering“ als neue Quelle von Spenderorganen: Eine systematische Übersichtsarbeit
Mußbach, F., Dahmen, U., Dirsch, O., & Settmacher, U. (2016) · Der Chirurg
Pospelov, A. D., Kutova, O. M., Efremov, Y. M., Nekrasova, A. A., Trushina, D. B., Gefter, S. D., Cherkasova, E. I., Timofeeva, L. B., Timashev, P. S., Zvyagin, A. V., et al. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4455127
Hydrogels and microfluidics: toward more physiologically relevant organ-on-a-chip models
Taromian, F., Mahmoudi, N., Aguilar, L. C., Stamp, M. E. M., Collins, D. J., & Nisbet, D. R. (2026) · Lab on a Chip · doi:10.1039/d6lc00628k
- Authors
- Mattei, G., Di Patria, V., Tirella, A., Alaimo, A., Elia, G., Corti, A., Paolicchi, A., & Ahluwalia, A.
- Affiliations
- University of Pisa (IT)
- Istituto di Fisiologia Clinica (IT)
- Corresponding author
- Mattei, G.
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 10
- Issue
- 2
- Pages
- 875-882
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W1975163366
- Cited by
- 78
- References
- 30
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 10 · 2023: 5 · 2022: 5 · 2021: 8 · 2020: 4 · 2019: 5 · 2018: 7 · 2017: 8 · 2016: 9 · 2015: 9 · 2014: 3
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- green
- zenodo.org
- Licence
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutial research information system (University of Pisa) (submittedVersion)
- Iris (University of Trento) (submittedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Funders
- European Commission
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- extracellular matrix, porcine liver, mechanical properties, compressive modulus, tissue engineering scaffolds, reproducibility
- MeSH terms
- Animals, Biomechanical Phenomena, Cell Survival, DNA, Extracellular Matrix, Liver, Reproducibility of Results, Cell Death, Imaging, Three-Dimensional, Sus scrofa, Tissue Scaffolds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
