The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis
Open accessAbstract
Rapid and simple-to-use diagnostic methods for tuberculosis are urgently needed. Recent development has unveiled the diagnostic power of the CRISPR system in the detection of viral infections. However, its potential use in detecting the Mycobacterium tuberculosis complex (MTB) remained unexplored. We developed a rapid CRISPR-based assay for TB detection and conducted a retrospective cohort study of 179 patients to evaluate the CRISPR-MTB test for identifying MTB in various forms of direct clinical samples. Its diagnostic performance was compared, in parallel with culture and the GeneXpert MTB/RIF assay (Xpert). The CRISPR-MTB test is highly sensitive with a near single-copy sensitivity, demands less sample input and offers shorter turnaround time than Xpert. When evaluated in the clinical cohort of both pulmonary and extra-pulmonary tuberculosis, the CRISPR-MTB test exhibited an overall improved sensitivity over both culture (79% vs 33%) and Xpert (79% vs 66%), without comprise in specificity (62/63, 98%). The CRISPR-MTB test exhibits an improved overall diagnostic performance over culture and Xpert across a variety of sample types, and offers great potential as a new diagnostic technique for both pulmonary and extra-pulmonary tuberculosis.
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aar6245 · Cited by 4,507
Correction of a genetic disease in mouse via use of CRISPR-Cas9
Wu, Y., Liang, D., Wang, Y., Bai, M., Tang, W., Bao, S., Yan, Z., Li, D., & Li, J. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.10.016 · Cited by 614 · cited 1×
“Cas9 was the first member of the Cas family which had been used for gene editing since 2013 [7,8].”
MethodRapid Detection of Mycobacterium tuberculosis by Recombinase Polymerase Amplification
Boyle, D. S., McNerney, R., Teng Low, H., Leader, B. T., Pérez-Osorio, A. C., Meyer, J. C., O'Sullivan, D. M., Brooks, D. G., Piepenburg, O., & Forrest, M. S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0103091 · Cited by 164
Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies
Aman, R., Mahas, A., & Mahfouz, M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00507 · Cited by 409
Metadata
- Authors
- Ai, J.-W., Zhou, X., Xu, T., Yang, M., Chen, Y., He, G.-Q., Pan, N., Cai, Y., Li, Y., Wang, X., Su, H., Wang, T., Zeng, W., & Zhang, W.-H.
- Venue
- Emerging Microbes & Infections
- Publisher
- Informa UK Limited
- Published
- 2019-01
- Volume
- 8
- Issue
- 1
- Pages
- 1361-1369
- ISSN
- 2222-1751 (ISSN-L)
- OpenAlex
- W2972417808
- Cited by
- 220
- References
- 4
- FWCI
- 14.41
- Type
- journal article
- Language
- en
- Open access
- gold
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- Ai, J.-W., Zhou, X., Xu, T., Yang, M., Chen, Y., He, G.-Q., Pan, N., Cai, Y., Li, Y., Wang, X., Su, H., Wang, T., Zeng, W., & Zhang, W.-H. (2019). CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis. Emerging Microbes & Infections. https://doi.org/10.1080/22221751.2019.1664939
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