The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Enhanced production and extracellular deposition of the endothelial-type plasminogen activator inhibitor in cultured human lung fibroblasts by transforming growth factor-beta.
Open accessAbstract
Cultured human embryonic lung fibroblasts were used as a model to study the effects of transforming growth factor-beta (TGF beta) on the plasminogen activator (PA) activity released by nontumorigenic cells into the culture medium. The cells were exposed to TGF beta under serum-free conditions, and the changes in PA activity and protein metabolism were analyzed by caseinolysis-in-agar assays, zymography, and polypeptide analysis. Treatment of the cells with TGF beta caused a significant decrease in the PA activity of the culture medium as analyzed by the caseinolysis-in-agar assays. The quantitatively most prominent effect of TGF beta on confluent cultures of cells was the induction of an Mr 47,000 protein, as detected by metabolic labeling. The Mr 47,000 protein was a PA inhibitor as judged by reverse zymography. It was antigenically related to a PA inhibitor secreted by HT-1080 tumor cells as demonstrated with monoclonal antibodies. The induced Mr 47,000 inhibitor was deposited into the growth substratum of the cells, as detected by metabolic labeling, immunoblotting analysis, and reverse zymography assays of extracellular matrix preparations. TGF beta also decreased the amounts of urokinase-type and tissue-type PAs accumulated in the conditioned medium, as detected by zymography. Epidermal growth factor antagonized the inhibitory effects of TGF beta by enhancing the amounts of the PAs. These results indicate that growth factors modulate the proteolytic balance of cultured cells by altering the amounts of PAs and their inhibitors.
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Cites
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Cited by
Edwards, D. R., Murphy, G., Reynolds, J. J., Whitham, S. E., Docherty, A. J., Angel, P., & Heath, J. K. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02449.x · Cited by 1,221
Suppression of experimental glomerulonephritis by antiserum against transforming growth factor β1
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Proteolytic activation of latent transforming growth factor-beta from fibroblast-conditioned medium.
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Metadata
- Authors
- Laiho, M., Saksela, O., Andreasen, P. A., & Keski-Oja, J.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1986-12-01
- Volume
- 103
- Issue
- 6
- Pages
- 2403-2410
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2033873544
- Cited by
- 437
- References
- 39
- FWCI
- 11.05
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Enhanced production and extracellular deposition of the endothelial-type plasminogen activator inhibitor in cultured human lung fibroblasts by transforming growth factor-beta. https://app.cochat.ai/papers/10.1083/jcb.103.6.2403
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- Laiho, M., Saksela, O., Andreasen, P. A., & Keski-Oja, J. (1986). Enhanced production and extracellular deposition of the endothelial-type plasminogen activator inhibitor in cultured human lung fibroblasts by transforming growth factor-beta. The Journal of cell biology. https://doi.org/10.1083/jcb.103.6.2403
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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