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A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum.
Open accessAbstract
We have devised a genetic selection for mutant yeast cells that fail to translocate secretory protein precursors into the lumen of the endoplasmic reticulum (ER). Mutant cells are selected by a procedure that requires a signal peptide-containing cytoplasmic enzyme chimera to remain in contact with the cytosol. This approach has uncovered a new secretory mutant, sec61, that is thermosensitive for growth and that accumulates multiple secretory and vacuolar precursor proteins that have not acquired any detectable posttranslational modifications associated with translocation into the ER. Preproteins that accumulate at the sec61 block sediment with the particulate fraction, but are exposed to the cytosol as judged by sensitivity to proteinase K. Thus, the sec61 mutation defines a gene that is required for an early cytoplasmic or ER membrane-associated step in protein translocation.
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Cited by
The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
Ye, Y., Meyer, H. H., & Rapoport, T. A. (2001) · Nature · doi:10.1038/414652a · Cited by 1,099
Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes.
Rapoport, T. A. (2007) · Nature · doi:10.1038/nature06384 · Cited by 977
Garcia-Bustos, J., Heitman, J., & Hall, M. N. (1991) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(91)90013-m · Cited by 549
Harding, T. M., Morano, K. A., Scott, S. V., & Klionsky, D. J. (1995) · The Journal of cell biology · doi:10.1083/jcb.131.3.591 · Cited by 503
Protein translocation across biological membranes
Wickner, W., & Schekman, R. (2005) · Science · doi:10.1126/science.1113752 · Cited by 458
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Metadata
- Authors
- Deshaies, R. J., & Schekman, R.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1987-08-01
- Volume
- 105
- Issue
- 2
- Pages
- 633-645
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2156163052
- Cited by
- 415
- References
- 0
- FWCI
- 11.04
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Deshaies, R. J., & Schekman, R. (1987). A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. The Journal of cell biology. https://doi.org/10.1083/jcb.105.2.633
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