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Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase.
Open accessAbstract
Microinjection of the catalytic subunit of cAMP-dependent protein kinase (A-kinase) into living fibroblasts or the treatment of these cells with agents that elevate the intracellular cAMP level caused marked alterations in cell morphology including a rounded phenotype and a complete loss of actin microfilament bundles. These effects were transient and fully reversible. Two-dimensional gel electrophoresis was used to analyze the changes in phosphoproteins from cells injected with A-kinase. These experiments showed that accompanying the disassembly of actin microfilaments, phosphorylation of myosin light chain kinase (MLCK) increased and concomitantly, the phosphorylation of myosin P-light chain decreased. Moreover, inhibiting MLCK activity via microinjection of affinity-purified antibodies specific to native MLCK caused a complete loss of microfilament bundle integrity and a decrease in myosin P-light chain phosphorylation, similar to that seen after injection of A-kinase. These data support the idea that A-kinase may regulate microfilament integrity through the phosphorylation and inhibition of MLCK activity in nonmuscle cells.
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Citations
Cited by
Focal Adhesions: Transmembrane Junctions Between the Extracellular Matrix and the Cytoskeleton
Burridge, K., Fath, K., Kelly, T., Nuckolls, G., & Turner, C. (1988) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.04.110188.002415 · Cited by 2,120
Blangy, A., Lane, H. A., d'Hérin, P., Harper, M., Kress, M., & Niggt, E. A. (1995) · Cell · doi:10.1016/0092-8674(95)90142-6 · Cited by 952
Dedicated Myosin Light Chain Kinases with Diverse Cellular Functions
Kamm, K. E., & Stull, J. T. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.r000028200 · Cited by 572
Lang, P., Gesbert, F., Delespine‐Carmagnat, M., Stancou, R., Pouchelet, M., & Bertoglio, J. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00383.x · Cited by 558
The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells
Riabowol, K., Draetta, G., Brizuela, L., Vandre, D., & Beach, D. (1989) · Cell · doi:10.1016/0092-8674(89)90914-8 · Cited by 425
Metadata
- Authors
- Lamb, N. J., Fernandez, A., Conti, M. A., Adelstein, R., Glass, D. B., Welch, W. J., & Feramisco, J. R.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1988-06-01
- Volume
- 106
- Issue
- 6
- Pages
- 1955-1971
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2103294240
- Cited by
- 274
- References
- 0
- FWCI
- 5.59
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase. https://app.cochat.ai/papers/10.1083/jcb.106.6.1955
- The paper
- Lamb, N. J., Fernandez, A., Conti, M. A., Adelstein, R., Glass, D. B., Welch, W. J., & Feramisco, J. R. (1988). Regulation of actin microfilament integrity in living nonmuscle cells by the cAMP-dependent protein kinase and the myosin light chain kinase. The Journal of cell biology. https://doi.org/10.1083/jcb.106.6.1955
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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