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Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes.
Open accessAbstract
We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give distinct staining patterns; the staining by anti-CC(1-30) is intracellular. This differential staining pattern is consistently observed in several systems, including cultured tumor cells; mouse embryonic, neonatal, and adult tissues; bovine fibropapillomas; and human colon carcinomas. The extracellular staining by anti-CC(1-30) partially resembles that seen with an antibody to fibronectin, suggesting that extracellular TGF-beta may be bound to matrix proteins. The intracellular staining by anti-LC(1-30) is similar to that seen with two other antibodies raised to peptides corresponding to either amino acids 266-278 of the TGF-beta 1 precursor sequence or to amino acids 50-75 of mature TGF-beta 1, suggesting that anti-LC(1-30) stains sites of TGF-beta synthesis. Results from RIA and ELISAs indicate that anti-LC(1-30) and anti-CC(1-30) recognize different epitopes of this peptide and of TGF-beta 1 itself.
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Cited by
Kulkarni, A. B., Huh, C. G., Becker, D., Geiser, A., Lyght, M., Flanders, K. C., Roberts, A. B., Sporn, M. B., Ward, J. M., & Karlsson, S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.2.770 · Cited by 1,967
Self-citationSuppression of experimental glomerulonephritis by antiserum against transforming growth factor β1
Border, W. A., Okuda, S., Languino, L. R., Sporn, M. B., & Ruoslahti, E. (1990) · Nature · doi:10.1038/346371a0 · Cited by 988
Self-citationYamamoto, T., Nakamura, T., Noble, N. A., Ruoslahti, E., & Border, W. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.1814 · Cited by 830
Broekelmann, T. J., Limper, A. H., Colby, T. V., & McDonald, J. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.15.6642 · Cited by 794
Frazier, K., Williams, S., Kothapalli, D., Klapper, H., & Grotendorst, G. R. (1996) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12363389 · Cited by 757
Metadata
- Authors
- Flanders, K. C., Thompson, N. L., Cissel, D. S., Van Obberghen-Schilling, E., Baker, C. C., Kass, M. E., Ellingsworth, L. R., Roberts, A. B., & Sporn, M. B.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1989-02-01
- Volume
- 108
- Issue
- 2
- Pages
- 653-660
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2099941826
- Cited by
- 370
- References
- 0
- FWCI
- 13.39
- Type
- journal article
- Language
- en
- Open access
- green
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- Flanders, K. C., Thompson, N. L., Cissel, D. S., Van Obberghen-Schilling, E., Baker, C. C., Kass, M. E., Ellingsworth, L. R., Roberts, A. B., & Sporn, M. B. (1989). Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes. The Journal of cell biology. https://doi.org/10.1083/jcb.108.2.653
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