The DOI matches its Crossref record.
Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes.
Open accessAbstract
We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give distinct staining patterns; the staining by anti-CC(1-30) is intracellular. This differential staining pattern is consistently observed in several systems, including cultured tumor cells; mouse embryonic, neonatal, and adult tissues; bovine fibropapillomas; and human colon carcinomas. The extracellular staining by anti-CC(1-30) partially resembles that seen with an antibody to fibronectin, suggesting that extracellular TGF-beta may be bound to matrix proteins. The intracellular staining by anti-LC(1-30) is similar to that seen with two other antibodies raised to peptides corresponding to either amino acids 266-278 of the TGF-beta 1 precursor sequence or to amino acids 50-75 of mature TGF-beta 1, suggesting that anti-LC(1-30) stains sites of TGF-beta synthesis. Results from RIA and ELISAs indicate that anti-LC(1-30) and anti-CC(1-30) recognize different epitopes of this peptide and of TGF-beta 1 itself.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Kulkarni, A. B., Huh, C. G., Becker, D., Geiser, A., Lyght, M., Flanders, K. C., Roberts, A. B., Sporn, M. B., Ward, J. M., & Karlsson, S. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.2.770 · Cited by 1,967
Self-citationSuppression of experimental glomerulonephritis by antiserum against transforming growth factor β1
Border, W. A., Okuda, S., Languino, L. R., Sporn, M. B., & Ruoslahti, E. (1990) · Nature · doi:10.1038/346371a0 · Cited by 988
Self-citationYamamoto, T., Nakamura, T., Noble, N. A., Ruoslahti, E., & Border, W. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.1814 · Cited by 830
Broekelmann, T. J., Limper, A. H., Colby, T. V., & McDonald, J. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.15.6642 · Cited by 794
Frazier, K., Williams, S., Kothapalli, D., Klapper, H., & Grotendorst, G. R. (1996) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12363389 · Cited by 757
The Transforming Growth Factor-βs
Roberts, A. B., & Sporn, M. B. (1990) · Handbook of Experimental Pharmacology · doi:10.1007/978-3-642-49295-2_8 · Cited by 746
Self-citationJoyce, M. E., Roberts, A. B., Sporn, M. B., & Bolander, M. E. (1990) · The Journal of cell biology · doi:10.1083/jcb.110.6.2195 · Cited by 733
Self-citationCui, W., Fowlis, D. J., Bryson, S., Duffie, E., Ireland, H., Balmain, A., & Akhurst, R. J. (1996) · Cell · doi:10.1016/s0092-8674(00)80127-0 · Cited by 628
Okuda, S., Languino, L. R., Ruoslahti, E., & Border, W. A. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114731 · Cited by 546
Khalil, N., O'Connor, R. N., Unruh, H. W., Warren, P. W., Flanders, K. C., Kemp, A., Bereznay, O. H., & Greenberg, A. H. (1991) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb/5.2.155 · Cited by 521
Self-citationKhalil, N., Bereznay, O., Sporn, M., & Greenberg, A. H. (1989) · The Journal of experimental medicine · doi:10.1084/jem.170.3.727 · Cited by 505
Self-citationHinz, B. (2016) · Experimental Eye Research · doi:10.1016/j.exer.2015.07.009 · Cited by 424
Lyons, K. M., Pelton, R. W., & Hogan, B. L. (1989) · Genes & Development · doi:10.1101/gad.3.11.1657 · Cited by 438
Transforming growth factor-beta activation in irradiated murine mammary gland.
Barcellos-Hoff, M. H., Derynck, R., Tsang, M. L., & Weatherbee, J. A. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117045 · Cited by 431
Sustained expression of TGF-β1 underlies development of progressive kidney fibrosis
Yamamoto, T., Noble, N. A., Miller, D. E., & Border, W. A. (1994) · Kidney International · doi:10.1038/ki.1994.122 · Cited by 405
Taipale, J., Lohi, J., Saarinen, J., Kovanen, P. T., & Keski-Oja, J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.9.4689 · Cited by 398
Expression of transforming growth factor-beta 1 is increased in human vascular restenosis lesions.
Nikol, S., Isner, J. M., Pickering, J. G., Kearney, M., Leclerc, G., & Weir, L. (1992) · Journal of Clinical Investigation · doi:10.1172/jci116027 · Cited by 375
Localization and actions of transforming growth factor-β s in the embryonic nervous system
Flanders, K. C., Lüdecke, G., Engels, S., Cissel, D. S., Roberts, A. B., Kondaiah, P., Lafyatis, R., Sporn, M. B., & Unsicker, K. (1991) · Development · doi:10.1242/dev.113.1.183 · Cited by 359
Self-citationTransforming growth factor-β1 is a new form of tumor suppressor with true haploid insufficiency
Tang, B., Böttinger, E. P., Jakowlew, S. B., Bagnall, K. M., Mariano, J., Anver, M. R., Letterio, J. J., & Wakefield, L. M. (1998) · Nature Medicine · doi:10.1038/nm0798-802 · Cited by 314
Logan, A., Berry, M., Gonzalez, A. M., Frautschy, S. A., Sporn, M. B., & Baird, A. (1994) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1994.tb00278.x · Cited by 297
Self-citationPotts, J. D., Dagle, J. M., Walder, J. A., Weeks, D. L., & Runyan, R. B. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.4.1516 · Cited by 260
Sterner-Kock, A., Thorey, I. S., Koli, K., Wempe, F., Otte, J., Bangsow, T., Kuhlmeier, K., Kirchner, T., Jin, S., Keski-Oja, J., et al. (2002) · Genes & Development · doi:10.1101/gad.229102 · Cited by 256
Sellheyer, K., Bickenbach, J. R., Rothnagel, J. A., Bundman, D., Longley, M. A., Krieg, T., Roche, N. S., Roberts, A. B., & Roop, D. R. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.11.5237 · Cited by 254
Self-citationA role for TGF-beta in oligodendrocyte differentiation.
McKinnon, R. D., Piras, G., Ida, J. A., & Dubois-Dalcq, M. (1993) · The Journal of cell biology · doi:10.1083/jcb.121.6.1397 · Cited by 248
Saika, S., Miyamoto, T., Yamanaka, O., Kato, T., Ohnishi, Y., Flanders, K. C., Ikeda, K., Nakajima, Y., Kao, W. W.-Y., Sato, M., et al. (2005) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)62357-7 · Cited by 102
Self-citationGrowth factor and procollagen type I gene expression in human liver disease
Malizia, G., Brunt, E. M., Peters, M. G., Rizzo, A., Broekelmann, T. J., & McDonald, J. A. (1995) · Gastroenterology · doi:10.1016/0016-5085(95)90019-5 · Cited by 89
Wang, Y., Moges, H., Bharucha, Y., & Symes, A. (2007) · Experimental Neurology · doi:10.1016/j.expneurol.2006.08.006 · Cited by 84
Metadata
- Authors
- Flanders, K. C., Thompson, N. L., Cissel, D. S., Van Obberghen-Schilling, E., Baker, C. C., Kass, M. E., Ellingsworth, L. R., Roberts, A. B., & Sporn, M. B.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1989-02-01
- Volume
- 108
- Issue
- 2
- Pages
- 653-660
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2099941826
- Cited by
- 370
- References
- 0
- FWCI
- 13.39
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
