The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast.
Open accessAbstract
Genes that function in translocation of secretory protein precursors into the ER have been identified by a genetic selection for mutant yeast cells that fail to translocate a signal peptide-cytosolic enzyme hybrid protein. The new mutants, sec62 and sec63, are thermosensitive for growth and accumulate a variety of soluble secretory and vacuolar precursors whose electrophoretic mobilities coincide with those of the corresponding in vitro translated polypeptides. Proteolytic sensitivity of precursor molecules in extracts of mutant cells confirms that polypeptide translocation is blocked. Some form of interaction among the SEC61 (Deshaies, R. J., and R. Schekman. 1987. J. Cell Biol. 105:633-645), SEC62 and SEC63 gene products is suggested by the observation that haploid cells containing any pair of the mutations are inviable at 24 degrees C and show a marked enhancement of the translocation defect. The translocation defects of two mutants (sec62 and sec63) have been reproduced in vitro. sec63 microsomes display low and thermolabile translocation activity for prepro-alpha-factor (pp alpha F) synthesized with a cytosol fraction from wild type yeast. These gene products may constitute part of the polypeptide recognition or translocation apparatus of the ER membrane. Pulse-chase analysis of the translocation-defective mutants demonstrates that insertion of pp alpha F into the ER can proceed posttranslationally.
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Cites
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Sec61p and BiP directly facilitate polypeptide translocation into the ER
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Metadata
- Authors
- Rothblatt, J. A., Deshaies, R. J., Sanders, S. L., Daum, G., & Schekman, R.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1989-12-01
- Volume
- 109
- Issue
- 6
- Pages
- 2641-2652
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2124761552
- Cited by
- 331
- References
- 43
- FWCI
- 10.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Rothblatt, J. A., Deshaies, R. J., Sanders, S. L., Daum, G., & Schekman, R. (1989). Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. The Journal of cell biology. https://doi.org/10.1083/jcb.109.6.2641
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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