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Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway

Novick, Peter, Field, Charles, Schekman, Randy·1980·Cell
DOI 10.1016/0092-8674(80)90128-2
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Abstract

Cells of a Saccharomyces cerevisiae mutant that is temperature-sensitive for secretion and cell surface growth become dense during incubation at the non-permissive temperature (37 degrees C). This property allows the selection of additional secretory mutants by sedimentation of mutagenized cells on a Ludox density gradient. Colonies derived from dense cells are screened for conditional growth and secretion of invertase and acid phosphatase. The sec mutant strains that accumulate an abnormally large intracellular pool of invertase at 37 degrees C (188 mutant clones) fall into 23 complementation groups, and the distribution of mutant alleles suggests that more complementation groups could be found. Bud emergence and incorporation of a plasma membrane sulfate permease activity stop quickly after a shift to 37 degrees C. Many of the mutants are thermoreversible; upon return to the permissive temperature (25 degrees C) the accumulated invertase is secreted. Electron microscopy of sec mutant cells reveals, with one exception, the temperature-dependent accumulation of membrane-enclosed secretory organelles. We suggest that these structures represent intermediates in a pathway in which secretion and plasma membrane assembly are colinear.

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Crossref · 10.1016/0092-8674(80)90128-2

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  • The exocyst is recruited to vesicle membranes by small GTPases and mediates the tethering of these vesicles to target membranes.

    “The exocyst is recruited to vesicle membranes by small GTPases and mediates the tethering of these vesicles to target membranes.”

    From Genome-scale CRISPR screening for modifiers of cellular LDL uptake (2021) · cites this paper as [21]

  • The exocyst is an octameric protein complex that was originally identified for its role in vesicular trafficking in budding yeast.

    “an octameric protein complex that was originally identified for its role in vesicular trafficking in budding yeast[20,21]”

    From Genome-scale CRISPR screening for modifiers of cellular LDL uptake (2021) · cites this paper as [21]

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Research works printed but not linked: 24

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Metadata

Authors
Novick, P., Field, C., & Schekman, R.
Venue
Cell
Publisher
Elsevier BV
Published
1980-08
Volume
21
Issue
1
Pages
205-215
ISSN
0092-8674
DOI
10.1016/0092-8674(80)90128-2
Cited by
1,449
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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