The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
Open accessAbstract
Taxol, a microtubule stabilizing drug, induces the formation of numerous microtubule asters in the cytoplasm of mitotic cells (De Brabander, M., G. Geuens, R. Nuydens, R. Willebrords, J. DeMey. 1981. Proc. Natl. Acad. Sci. USA. 78:5608-5612). The center of these asters share with spindle poles some characteristics such as the presence of centrosomal material and calmodulin. We have recently reproduced the assembly of taxol asters in a cell-free system (Buendia, B., C. Antony, F. Verde, M. Bornens, and E. Karsenti. 1990. J. Cell Sci. 97:259-271) using extracts of Xenopus eggs. In this paper, we show that taxol aster assembly requires phosphorylation, and that they do not grow from preformed centers, but rather by a reorganization of microtubules first crosslinked into bundles. This process seems to involve sliding of microtubules along each other and we show that cytoplasmic dynein is required for taxol aster assembly. This result provides a possible functional basis to the recent findings, that dynein is present in the spindle and enriched near spindle poles (Pfarr, C. M., M. Cove, P. M. Grissom, T. S. Hays, M. E. Porter, and J. R. McIntosh. 1990. Nature (Lond.). 345:263-265; Steuer, E. R., L. Wordeman, T. A. Schroer, and M. P. Sheetz. 1990. Nature (Lond.). 345:266-268).
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Citations
Cites
Promotion of microtubule assembly in vitro by taxol
SCHIFF, P. B., FANT, J., & HORWITZ, S. B. (1979) · Nature · doi:10.1038/277665a0 · Cited by 3,600
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
VALE, R., REESE, T., & SHEETZ, M. (1985) · Cell · doi:10.1016/s0092-8674(85)80099-4 · Cited by 1,993 · cited 2×
“Kinesin promotes plus end-directed motility (Porter et al., 1987; Vale et al., 1985), whereas dynein promotes minus end-directed movement (Paschal and Vallee, 1987; Schroer et al.”
Background
Cited by
Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas
Porter, M. E., Bower, R., Knott, J. A., Byrd, P., & Dentler, W. (1999) · Molecular Biology of the Cell · doi:10.1091/mbc.10.3.693 · Cited by 333
Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly
Wiese, C., Wilde, A., Moore, M. S., Adam, S. A., Merdes, A., & Zheng, Y. (2001) · Science · doi:10.1126/science.1057661 · Cited by 323
Opposing motor activities are required for the organization of the mammalian mitotic spindle pole.
Gaglio, T., Saredi, A., Bingham, J. B., Hasbani, M. J., Gill, S. R., Schroer, T. A., & Compton, D. A. (1996) · The Journal of cell biology · doi:10.1083/jcb.135.2.399 · Cited by 290
NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.
Goh, P. Y., & Kilmartin, J. V. (1993) · The Journal of cell biology · doi:10.1083/jcb.121.3.503 · Cited by 264
Giet, R., Uzbekov, R., Cubizolles, F., Le Guellec, K., & Prigent, C. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.21.15005 · Cited by 219
Metadata
- Authors
- Verde, F., Berrez, J. M., Antony, C., & Karsenti, E.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1991-03-15
- Volume
- 112
- Issue
- 6
- Pages
- 1177-1187
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2143708956
- Cited by
- 289
- References
- 2
- FWCI
- 7.36
- Type
- journal article
- Language
- en
- Open access
- green
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- Verde, F., Berrez, J. M., Antony, C., & Karsenti, E. (1991). Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein. The Journal of cell biology. https://doi.org/10.1083/jcb.112.6.1177
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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