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Cited by 168Bone Metabolism and DiseasesBone and Dental Protein StudiesCell Adhesion Molecules Research

The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.

Vitronectin receptor has a role in bone resorption but does not mediate tight sealing zone attachment of osteoclasts to the bone surface.

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Lakkakorpi, P T, Horton, M A, Helfrich, M H, Karhukorpi, E K, Väänänen, H K·1991·The Journal of cell biology
DOI 10.1083/jcb.115.4.1179PMC PMC2289948
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Abstract

During bone resorption, osteoclasts form a tight attachment, the sealing zone, around resorption lacunae. Vitronectin receptor has previously been shown to be expressed in osteoclasts and it has been suggested that it mediates the tight attachment at the sealing zone. In this study we have shown that glycine-arginine-glycine-aspartic acid-serine pentapeptide inhibits bone resorption by isolated osteoclasts and drastically changes the morphology of the osteoclasts. When the vitronectin receptor was localized by immunofluorescence in rat and chicken osteoclasts cultured on bone slices, it was found to be distributed throughout the osteoclast cell membrane except in the sealing zone areas. Immunoperoxidase staining of rat bone sections at the light microscopical level also revealed intense staining of the cell membrane with occasional small unstained areas, probably corresponding to the sealing zones. Immunoelectron microscopy confirmed the results obtained by light microscopy showing specific labeling only at the ruffled borders and basolateral membranes (0.82 and 2.43 gold particles/microns of membrane, respectively), but not at the sealing zone areas (0.06 gold particles/microns of membrane). Both alpha v and beta 3 subunits of the vitronectin receptor were similarly localized. These results strongly suggest that, although the vitronectin receptor is important in the function of osteoclasts, it is not mediating the final sealing zone attachment of the osteoclasts to the mineralized bone surface.

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Metadata

Authors
Lakkakorpi, P. T., Horton, M. A., Helfrich, M. H., Karhukorpi, E. K., & Väänänen, H. K.
Affiliations
University of Oulu (FI)
ORCID
Venue
The Journal of cell biology
Publisher
Rockefeller University Press
Published
1991-11-15
Volume
115
Issue
4
Pages
1179-1186
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.115.4.1179
PMC
PMC2289948
OpenAlex
W2067074361
Cited by
168
References
33
Citations by year
2024: 2 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 1 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 1
FWCI
3.70
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
integrin αvβ3, osteoclasts, bone resorption, sealing zone, integrin αvβ5, ruffled border, basolateral membrane, osteoclast morphology
MeSH terms
Animals, Bone and Bones, Bone Resorption, Cell Adhesion, Chickens, Fluorescent Antibody Technique, Osteoclasts, Receptors, Immunologic, Integrins, Microscopy, Immunoelectron, Receptors, Vitronectin, Rats

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