The DOI matches its Crossref record; 73 of the 73 references it cites were found in a registry or an index.
Latent transforming growth factor-beta 1 associates to fibroblast extracellular matrix via latent TGF-beta binding protein
Open accessAbstract
The role of latent transforming growth factor-beta (TGF-beta) binding protein (LTBP) in the association of TGF-beta 1 to the extracellular matrix of cultured fibroblasts and HT-1080 fibrosarcoma cells was studied by immunochemical methods. The matrices were isolated from the cells, and the levels of LTBP and TGF-beta 1 were estimated by immunoblotting and immunoprecipitation. LTBP, TGF-beta 1, and its propeptide (latency-associated peptide, LAP) were found to associate to the extracellular matrix. Immunoblotting analysis indicated that treatment of the cells with plasmin resulted in a concomitant time and dose dependent release of both LTBP and TGF-beta 1 from the extracellular matrix to the supernatant. Comparison of molecular weights suggested that plasmin treatment resulted in the cleavage of LTBP from the high molecular weight fibroblast form to a form resembling the low molecular weight LTBP found in platelets. Pulse-chase and immunoprecipitation analysis indicated that both the free form of LTBP and LTBP complexed to latent TGF-beta were efficiently incorporated in the extracellular matrix, from where both complexes were slowly released to the culture medium. Addition of plasmin to the chase solution resulted, however, in a rapid release of LTBP from the matrix. Fibroblast derived LTBP was found to associate to the matrix of HT-1080 cells in a plasmin sensitive manner as shown by immunoprecipitation analysis. These results suggest that the latent form of TGF-beta 1 associates with the extracellular matrix via LTBP, and that the release of latent TGF-beta 1 from the matrix is a consequence of proteolytic cleavage(s) of LTBP.
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Cited by
TGF-β and the TGF-β family: context-dependent roles in cell and tissue physiology
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Self-citationIntegrins and the activation of latent transforming growth factor β1 – An intimate relationship
Wipff, P.-J., & Hinz, B. (2008) · European Journal of Cell Biology · doi:10.1016/j.ejcb.2008.01.012 · Cited by 517
TGF-β – an excellent servant but a bad master
Kubiczkova, L., Sedlarikova, L., Hajek, R., & Sevcikova, S. (2012) · Journal of Translational Medicine · doi:10.1186/1479-5876-10-183 · Cited by 511
Transforming growth factor‐β in stem cells and tissue homeostasis
Xu, X., Zheng, L., Yuan, Q., Zhen, G., Crane, J. L., Zhou, X., & Cao, X. (2018) · Bone Research · doi:10.1038/s41413-017-0005-4 · Cited by 414
Poltavets, V., Kochetkova, M., Pitson, S. M., & Samuel, M. S. (2018) · Frontiers in Oncology · doi:10.3389/fonc.2018.00431 · Cited by 377
Metadata
- Authors
- Taipale, J., Miyazono, K., Heldin, C., & Keski-Oja, J.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1994-01-01
- Volume
- 124
- Issue
- 1
- Pages
- 171-181
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2166224134
- Cited by
- 448
- References
- 73
- FWCI
- 6.99
- Type
- journal article
- Language
- en
- Open access
- green
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- Taipale, J., Miyazono, K., Heldin, C., & Keski-Oja, J. (1994). Latent transforming growth factor-beta 1 associates to fibroblast extracellular matrix via latent TGF-beta binding protein. The Journal of cell biology. https://doi.org/10.1083/jcb.124.1.171
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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