Characteristics of endoplasmic reticulum-derived transport vesicles.
Open accessAbstract
We have isolated vesicles that mediate protein transport from the ER to Golgi membranes in perforated yeast. These vesicles, which form de novo during in vitro incubations, carry lumenal and membrane proteins that include core-glycosylated pro-alpha-factor, Bet1, Sec22, and Bos1, but not ER-resident Kar2 or Sec61 proteins. Thus, lumenal and membrane proteins in the ER are sorted prior to transport vesicle scission. Inhibition of Ypt1p-function, which prevents newly formed vesicles from docking to cis-Golgi membranes, was used to block transport. Vesicles that accumulate are competent for fusion with cis-Golgi membranes, but not with ER membranes, and thus are functionally committed to vectorial transport. A 900-fold enrichment was developed using differential centrifugation and a series of velocity and equilibrium density gradients. Electron microscopic analysis shows a uniform population of 60 nm vesicles that lack peripheral protein coats. Quantitative Western blot analysis indicates that protein markers of cytosol and cellular membranes are depleted throughout the purification, whereas the synaptobrevin-like Bet1, Sec22, and Bos1 proteins are highly enriched. Uncoated ER-derived transport vesicles (ERV) contain twelve major proteins that associate tightly with the membrane. The ERV proteins may represent abundant cargo and additional targeting molecules.
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PS1 may be localized selectively in the ER-Golgi intermediate compartment involved in intracellular protein trafficking.
“selectively in the ER-Golgi intermediate compartment involved in intracellular protein trafficking (Hauri and Schweizer, 1992;Rexach et al., 1994;Krijnse-Locker et al., 1995).”
From Light and Electron Microscopic Localization of Presenilin-1 in Primate Brain (1997) · cites this paper as Rexach et al., 1994
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Light and Electron Microscopic Localization of Presenilin-1 in Primate Brain
Lah, J. J., Heilman, C. J., Nash, N. R., Rees, H. D., Yi, H., Counts, S. E., & Levey, A. I. (1997) · The Journal of Neuroscience · doi:10.1523/jneurosci.17-06-01971.1997 · Cited by 168 · see citing works
- Authors
- Rexach, M. F., Latterich, M., & Schekman, R. W.
- Affiliations
- University of California, Berkeley (US)
- ORCID
- Schekman, R W
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1994-09-01
- Volume
- 126
- Issue
- 5
- Pages
- 1133-1148
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2120166
- OpenAlex
- W1978299049
- Cited by
- 114
- References
- 63
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2021: 2 · 2018: 1 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- endoplasmic reticulum, ER-Golgi transport, vesicle scission, Ypt1p, vesicle fusion, protein sorting, vesicle purification
- MeSH terms
- Biological Transport, Cell Compartmentation, Endoplasmic Reticulum, Fungal Proteins, Golgi Apparatus, Intracellular Membranes, Membrane Fusion, Membrane Glycoproteins, Saccharomyces cerevisiae, In Vitro Techniques
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