The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles
Open accessAbstract
The occurrence of clathrin-coated buds on immature granules (IGs) of the regulated secretory pathway suggests that specific transmembrane proteins are sorted into these buds through interaction with cytosolic adaptor proteins. By quantitative immunoelectron microscopy of rat endocrine pancreatic β cells and exocrine parotid and pancreatic cells, we show for the first time that the mannose 6–phosphate receptors (MPRs) for lysosomal enzyme sorting colocalize with the AP-1 adaptor in clathrin-coated buds on IGs. Furthermore, the concentrations of both MPR and AP-1 decline by ∼90% as the granules mature. Concomitantly, in exocrine secretory cells lysosomal proenzymes enter and then are sorted out of IGs, just as was previously observed in β cells (Kuliawat, R., J. Klumperman, T. Ludwig, and P. Arvan. 1997. J. Cell Biol. 137:595–608). The exit of MPRs in AP-1/clathrin-coated buds is selective, indicated by the fact that the membrane protein phogrin is not removed from maturing granules. We have also made the first observation of a soluble N-ethylmaleimide–sensitive factor attachment protein receptor, syntaxin 6, which has been implicated in clathrin-coated vesicle trafficking from the TGN to endosomes (Bock, J.B., J. Klumperman, S. Davanger, and R.H. Scheller. 1997. Mol. Biol. Cell. 8:1261–1271) that enters and then exits the regulated secretory pathway during granule maturation. Thus, we hypothesize that during secretory granule maturation, MPR–ligand complexes and syntaxin 6 are removed from IGs by AP-1/clathrin-coated vesicles, and then delivered to endosomes.
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Self-citationCLATHRIN-COATED VESICLE FORMATION AND PROTEIN SORTING: An Integrated Process
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Cited by
Burgoyne, R. D., & Morgan, A. (2003) · Physiological Reviews · doi:10.1152/physrev.00031.2002 · Cited by 934
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor
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Functions of adaptor protein (AP)-3 and AP-1 in tyrosinase sorting from endosomes to melanosomes
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The Rab27a/Granuphilin Complex Regulates the Exocytosis of Insulin-Containing Dense-Core Granules
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Metadata
- Authors
- Klumperman, J., Kuliawat, R., Griffith, J. M., Geuze, H. J., & Arvan, P.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1998-04-20
- Volume
- 141
- Issue
- 2
- Pages
- 359-371
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2052542184
- Cited by
- 287
- References
- 46
- FWCI
- 8.77
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles. https://app.cochat.ai/papers/10.1083/jcb.141.2.359
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- Klumperman, J., Kuliawat, R., Griffith, J. M., Geuze, H. J., & Arvan, P. (1998). Mannose 6–Phosphate Receptors Are Sorted from Immature Secretory Granules via Adaptor Protein AP-1, Clathrin, and Syntaxin 6–positive Vesicles. The Journal of Cell Biology. https://doi.org/10.1083/jcb.141.2.359
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