The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles
Open accessAbstract
Previous genetic and biochemical studies have led to the hypothesis that the essential mitotic bipolar kinesin, KLP61F, cross-links and slides microtubules (MTs) during spindle assembly and function. Here, we have tested this hypothesis by immunofluorescence and immunoelectron microscopy (immunoEM). We show that Drosophila embryonic spindles at metaphase and anaphase contain abundant bundles of MTs running between the spindle poles. These interpolar MT bundles are parallel near the poles and antiparallel in the midzone. We have observed that KLP61F motors, phosphorylated at a cdk1/cyclin B consensus domain within the BimC box (BCB), localize along the length of these interpolar MT bundles, being concentrated in the midzone region. Nonphosphorylated KLP61F motors, in contrast, are excluded from the spindle and display a cytoplasmic localization. Immunoelectron microscopy further suggested that phospho-KLP61F motors form cross-links between MTs within interpolar MT bundles. These bipolar KLP61F MT-MT cross-links should be capable of organizing parallel MTs into bundles within half spindles and sliding antiparallel MTs apart in the spindle midzone. Thus we propose that bipolar kinesin motors and MTs interact by a “sliding filament mechanism” during the formation and function of the mitotic spindle.
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Cited by
The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
Kapitein, L. C., Peterman, E. J. G., Kwok, B. H., Kim, J. H., Kapoor, T. M., & Schmidt, C. F. (2005) · Nature · doi:10.1038/nature03503 · Cited by 678
Sharp, D. J., Rogers, G. C., & Scholey, J. M. (2000) · Nature · doi:10.1038/35024000 · Cited by 592
Self-citationTraffic control: regulation of kinesin motors
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Centrosome Reduction During Gametogenesis and Its Significance1
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Metadata
- Authors
- Sharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., & Scholey, J. M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-01-11
- Volume
- 144
- Issue
- 1
- Pages
- 125-138
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2108422673
- Cited by
- 313
- References
- 44
- FWCI
- 5.01
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Sharp, D. J., McDonald, K. L., Brown, H. M., Matthies, H. J., Walczak, C., Vale, R. D., Mitchison, T. J., & Scholey, J. M. (1999). The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles. The Journal of Cell Biology. https://doi.org/10.1083/jcb.144.1.125
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