The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
The Junction-associated Protein AF-6 Interacts and Clusters with Specific Eph Receptor Tyrosine Kinases at Specialized Sites of Cell–Cell Contact in the Brain
Open accessAbstract
The AF-6/afadin protein, which contains a single PDZ domain, forms a peripheral component of cell membranes at specialized sites of cell–cell junctions. To identify potential receptor-binding targets of AF-6 we screened the PDZ domain of AF-6 against a range of COOH-terminal peptides selected from receptors having potential PDZ domain-binding termini. The PDZ domain of AF-6 interacts with a subset of members of the Eph subfamily of RTKs via its COOH terminus both in vitro and in vivo. Cotransfection of a green fluorescent protein-tagged AF-6 fusion protein with full-length Eph receptors into heterologous cells induces a clustering of the Eph receptors and AF-6 at sites of cell–cell contact. Immunohistochemical analysis in the adult rat brain reveals coclustering of AF-6 with Eph receptors at postsynaptic membrane sites of excitatory synapses in the hippocampus. Furthermore, AF-6 is a substrate for a subgroup of Eph receptors and phosphorylation of AF-6 is dependent on a functional kinase domain of the receptor. The physical interaction of endogenous AF-6 with Eph receptors is demonstrated by coimmunoprecipitation from whole rat brain lysates. AF-6 is a candidate for mediating the clustering of Eph receptors at postsynaptic specializations in the adult rat brain.
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Cites
Occludin: a novel integral membrane protein localizing at tight junctions.
Furuse, M., Hirase, T., Itoh, M., Nagafuchi, A., Yonemura, S., Tsukita, S., & Tsukita, S. (1993) · The Journal of cell biology · doi:10.1083/jcb.123.6.1777 · Cited by 2,580
Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ Domains
Songyang, Z., Fanning, A. S., Fu, C., Xu, J., Marfatia, S. M., Chishti, A. H., Crompton, A., Chan, A. C., Anderson, J. M., & Cantley, L. C. (1997) · Science · doi:10.1126/science.275.5296.73 · Cited by 1,385
Cited by
González-Mariscal, L., Betanzos, A., Nava, P., & Jaramillo, B. E. (2003) · Progress in Biophysics and Molecular Biology · doi:10.1016/s0079-6107(02)00037-8 · Cited by 1,160
Mechanisms and functions of eph and ephrin signalling
Kullander, K., & Klein, R. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm856 · Cited by 1,156
Gale, N. W., & Yancopoulos, G. D. (1999) · Genes & Development · doi:10.1101/gad.13.9.1055 · Cited by 784
Cell adhesion molecules: signalling functions at the synapse
Dalva, M. B., McClelland, A. C., & Kayser, M. S. (2007) · Nature Reviews Neuroscience · doi:10.1038/nrn2075 · Cited by 620
Penzes, P., Beeser, A., Chernoff, J., Schiller, M. R., Eipper, B. A., Mains, R. E., & Huganir, R. L. (2003) · Neuron · doi:10.1016/s0896-6273(02)01168-6 · Cited by 466
Metadata
- Authors
- Buchert, M., Schneider, S., Meskenaite, V., Adams, M. T., Canaani, E., Baechi, T., Moelling, K., & Hovens, C. M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-01-25
- Volume
- 144
- Issue
- 2
- Pages
- 361-371
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2157726561
- Cited by
- 195
- References
- 2
- FWCI
- 7.22
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: The Junction-associated Protein AF-6 Interacts and Clusters with Specific Eph Receptor Tyrosine Kinases at Specialized Sites of Cell–Cell Contact in the Brain. https://app.cochat.ai/papers/10.1083/jcb.144.2.361
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- Buchert, M., Schneider, S., Meskenaite, V., Adams, M. T., Canaani, E., Baechi, T., Moelling, K., & Hovens, C. M. (1999). The Junction-associated Protein AF-6 Interacts and Clusters with Specific Eph Receptor Tyrosine Kinases at Specialized Sites of Cell–Cell Contact in the Brain. The Journal of Cell Biology. https://doi.org/10.1083/jcb.144.2.361
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