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Establishment of the Dorsal–Ventral Axis in Xenopus Embryos Coincides with the Dorsal Enrichment of Dishevelled That Is Dependent on Cortical Rotation
Open accessAbstract
Examination of the subcellular localization of Dishevelled (Dsh) in fertilized Xenopus eggs revealed that Dsh is associated with vesicle-like organelles that are enriched on the prospective dorsal side of the embryo after cortical rotation. Dorsal enrichment of Dsh is blocked by UV irradiation of the vegetal pole, a treatment that inhibits development of dorsal cell fates, linking accumulation of Dsh and specification of dorsal cell fates. Investigation of the dynamics of Dsh localization using Dsh tagged with green fluorescent protein (Dsh-GFP) demonstrated that Dsh-GFP associates with small vesicle-like organelles that are directionally transported along the parallel array of microtubules towards the prospective dorsal side of the embryo during cortical rotation. Perturbing the assembly of the microtubule array with D2O, a treatment that promotes the random assembly of the array and the dorsalization of embryos, randomizes translocation of Dsh-GFP. Conversely, UV irradiation of the vegetal pole abolishes movement of Dsh-GFP. Finally, we demonstrate that overexpression of Dsh can stabilize β-catenin in Xenopus. These data suggest that the directional translocation of Dsh along microtubules during cortical rotation and its subsequent enrichment on the prospective dorsal side of the embryo play a role in locally activating a maternal Wnt pathway responsible for establishing dorsal cell fates in Xenopus.
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Citations
Cited by
Dishevelled controls cell polarity during Xenopus gastrulation
Wallingford, J. B., Rowning, B. A., Vogeli, K. M., Rothbächer, U., Fraser, S. E., & Harland, R. M. (2000) · Nature · doi:10.1038/35011077 · Cited by 751
Self-citationDORSAL-VENTRAL PATTERNING AND NEURAL INDUCTION IN XENOPUS EMBRYOS
De Robertis, E. M., & Kuroda, H. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.011403.154124 · Cited by 671
Kühl, M., Sheldahl, L. C., Malbon, C. C., & Moon, R. T. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.17.12701 · Cited by 517
Self-citationTao, Q., Yokota, C., Puck, H., Kofron, M., Birsoy, B., Yan, D., Asashima, M., Wylie, C. C., Lin, X., & Heasman, J. (2005) · Cell · doi:10.1016/j.cell.2005.01.013 · Cited by 510
The developmental biology of Dishevelled: an enigmatic protein governing cell fate and cell polarity
Wallingford, J. B., & Habas, R. (2005) · Development · doi:10.1242/dev.02068 · Cited by 509
Metadata
- Authors
- Miller, J. R., Rowning, B. A., Larabell, C. A., Yang-Snyder, J. A., Bates, R. L., & Moon, R. T.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-07-26
- Volume
- 146
- Issue
- 2
- Pages
- 427-438
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2107745042
- Cited by
- 254
- References
- 0
- FWCI
- 7.18
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Establishment of the Dorsal–Ventral Axis in Xenopus Embryos Coincides with the Dorsal Enrichment of Dishevelled That Is Dependent on Cortical Rotation. https://app.cochat.ai/papers/10.1083/jcb.146.2.427
- The paper
- Miller, J. R., Rowning, B. A., Larabell, C. A., Yang-Snyder, J. A., Bates, R. L., & Moon, R. T. (1999). Establishment of the Dorsal–Ventral Axis in Xenopus Embryos Coincides with the Dorsal Enrichment of Dishevelled That Is Dependent on Cortical Rotation. The Journal of Cell Biology. https://doi.org/10.1083/jcb.146.2.427
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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