The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
The 193-Kd Vault Protein, Vparp, Is a Novel Poly(Adp-Ribose) Polymerase
Open accessAbstract
Mammalian vaults are ribonucleoprotein (RNP) complexes, composed of a small ribonucleic acid and three proteins of 100, 193, and 240 kD in size. The 100-kD major vault protein (MVP) accounts for >70% of the particle mass. We have identified the 193-kD vault protein by its interaction with the MVP in a yeast two-hybrid screen and confirmed its identity by peptide sequence analysis. Analysis of the protein sequence revealed a region of ∼350 amino acids that shares 28% identity with the catalytic domain of poly(ADP-ribose) polymerase (PARP). PARP is a nuclear protein that catalyzes the formation of ADP-ribose polymers in response to DNA damage. The catalytic domain of p193 was expressed and purified from bacterial extracts. Like PARP, this domain is capable of catalyzing a poly(ADP-ribosyl)ation reaction; thus, the 193-kD protein is a new PARP. Purified vaults also contain the poly(ADP-ribosyl)ation activity, indicating that the assembled particle retains enzymatic activity. Furthermore, we show that one substrate for this vault-associated PARP activity is the MVP. Immunofluorescence and biochemical data reveal that p193 protein is not entirely associated with the vault particle, suggesting that it may interact with other protein(s). A portion of p193 is nuclear and localizes to the mitotic spindle.
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Cites
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van Steensel, B., & de Lange, T. (1997) · Nature · doi:10.1038/385740a0 · Cited by 1,219
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A superfamily of conserved domains in DNA damage‐ responsive cell cycle checkpoint proteins
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Metadata
- Authors
- Kickhoefer, V. A., Siva, A. C., Kedersha, N. L., Inman, E. M., Ruland, C., Streuli, M., & Rome, L. H.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-09-06
- Volume
- 146
- Issue
- 5
- Pages
- 917-928
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2025550674
- Cited by
- 405
- References
- 3
- FWCI
- 10.55
- Type
- journal article
- Language
- en
- Open access
- green
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- Kickhoefer, V. A., Siva, A. C., Kedersha, N. L., Inman, E. M., Ruland, C., Streuli, M., & Rome, L. H. (1999). The 193-Kd Vault Protein, Vparp, Is a Novel Poly(Adp-Ribose) Polymerase. The Journal of Cell Biology. https://doi.org/10.1083/jcb.146.5.917
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