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Cited by 199Cell Adhesion Molecules ResearchBarrier Structure and Function StudiesCellular Mechanics and Interactions

Monocytes Induce Reversible Focal Changes in Vascular Endothelial Cadherin Complex during Transendothelial Migration under Flow

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Allport, Jennifer R., Muller, William A., Luscinskas, Francis W.·2000·The Journal of Cell Biology
DOI 10.1083/jcb.148.1.203PMC PMC2156206
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Abstract

The vascular endothelial cell cadherin complex (VE-cadherin, alpha-, beta-, and gamma-catenin, and p120/p100) localizes to adherens junctions surrounding vascular endothelial cells and may play a critical role in the transendothelial migration of circulating blood leukocytes. Previously, we have reported that neutrophil adhesion to human umbilical vein endothelial cell (HUVEC) monolayers, under static conditions, results in a dramatic loss of the VE-cadherin complex. Subsequent studies by us and others (Moll, T., E. Dejana, and D. Vestweber. 1998. J. Cell Biol. 140:403-407) suggested that this phenomenon might reflect degradation by neutrophil proteases released during specimen preparation. We postulated that some form of disruption of the VE-cadherin complex might, nonetheless, be a physiological process during leukocyte transmigration. In the present study, the findings demonstrate a specific, localized effect of migrating leukocytes on the VE-cadherin complex in cytokine-activated HUVEC monolayers. Monocytes and in vitro differentiated U937 cells induce focal loss in the staining of VE-cadherin, alpha-catenin, beta-catenin, and plakoglobin during transendothelial migration under physiological flow conditions. These events are inhibited by antibodies that prevent transendothelial migration and are reversed following transmigration. Together, these data suggest that an endothelial-dependent step of transient and focal disruption of the VE-cadherin complex occurs during leukocyte transmigration.

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  • Adherens junctions regulate transendothelial migration of lymphocytes, monocytes, and neutrophils.

    “AJs are connected to cytoskeleton, modulate receptor signaling (203), and regulate transendothelial migration of lymphocytes (601), monocytes (12, 203), and neutrophils (642).”

    From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [12]

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Research works printed but not linked: 3

  1. Lampugnani M.G. Corada M. Caveda L. Breviario F. Ayalon O. Geiger B. Dejana E. The molecular organization of endothelial cell to cell junctionsdifferential association of plakoglobin, β-catenin, and α-catenin with vascular endothelial cadherin (VE-cadherin) J. Cell Biol. 129 1995 203 218 7698986 PMC2120375
  2. Luscinskas F.W. Ding H. Lichtman A.H. P-selectin and VCAM-1 mediate rolling and arrest of CD4+ T-lymphocytes on TNF-α–activated vascular endothelium under flow J. Exp. Med. 181 1995 1179 1186 7532680 PMC2191919
  3. Newman P.J. Switched at birtha new family for PECAM-1 J. Clin. Invest. 103 1999 5 9 9884328 PMC407871

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Authors
Allport, J. R., Muller, W. A., & Luscinskas, F. W.
Affiliations
  • Brigham and Women's Hospital (US)
  • bDepartment of Pathology, Weill Medical College of Cornell University, New York, New York 10021
ORCID
Luscinskas, Francis W.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2000-01-10
Volume
148
Issue
1
Pages
203-216
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.148.1.203
PMC
PMC2156206
OpenAlex
W2027826658
Cited by
199
References
45
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 6 · 2014: 8 · 2013: 7 · 2012: 6
FWCI
9.70
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (R01HL046849, HL-53993, HL 46849, P01HL036028, HL36028, R01HL053993)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
VE-cadherin, adherens junctions, monocytes, leukocyte transendothelial migration, alpha-catenin, β-catenin, plakoglobin, human umbilical vein endothelial cells, U937 cells, endothelial cell adhesion
MeSH terms
Cadherin 5, Cell Movement, Cells, Cultured, Cytoskeletal Proteins, Endothelium, Vascular, Humans, Monocytes, Staining and Labeling, Trans-Activators, Antigens, CD, Cadherins, Lipopolysaccharide Receptors, Platelet Endothelial Cell Adhesion Molecule-1, U937 Cells, beta Catenin, alpha Catenin

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