The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Actin-Dependent Propulsion of Endosomes and Lysosomes by Recruitment of N-Wasp✪
Open accessAbstract
We examined the spatial and temporal control of actin assembly in living Xenopus eggs. Within minutes of egg activation, dynamic actin-rich comet tails appeared on a subset of cytoplasmic vesicles that were enriched in protein kinase C (PKC), causing the vesicles to move through the cytoplasm. Actin comet tail formation in vivo was stimulated by the PKC activator phorbol myristate acetate (PMA), and this process could be reconstituted in a cell-free system. We used this system to define the characteristics that distinguish vesicles associated with actin comet tails from other vesicles in the extract. We found that the protein, N-WASP, was recruited to the surface of every vesicle associated with an actin comet tail, suggesting that vesicle movement results from actin assembly nucleated by the Arp2/3 complex, the immediate downstream target of N-WASP. The motile vesicles accumulated the dye acridine orange, a marker for endosomes and lysosomes. Furthermore, vesicles associated with actin comet tails had the morphological features of multivesicular endosomes as revealed by electron microscopy. Endosomes and lysosomes from mammalian cells preferentially nucleated actin assembly and moved in the Xenopus egg extract system. These results define endosomes and lysosomes as recruitment sites for the actin nucleation machinery and demonstrate that actin assembly contributes to organelle movement. Conversely, by nucleating actin assembly, intracellular membranes may contribute to the dynamic organization of the actin cytoskeleton.
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Cited by
Actin Dynamics, Architecture, and Mechanics in Cell Motility
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A Modular Design for the Clathrin- and Actin-Mediated Endocytosis Machinery
Kaksonen, M., Toret, C. P., & Drubin, D. G. (2005) · Cell · doi:10.1016/j.cell.2005.09.024 · Cited by 726
Kinesin Superfamily Motor Protein KIF17 and mLin-10 in NMDA Receptor-Containing Vesicle Transport
Setou, M., Nakagawa, T., Seog, D.-H., & Hirokawa, N. (2000) · Science · doi:10.1126/science.288.5472.1796 · Cited by 708
Harnessing actin dynamics for clathrin-mediated endocytosis
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Metadata
- Authors
- Taunton, J., Rowning, B. A., Coughlin, M. L., Wu, M., Moon, R. T., Mitchison, T. J., & Larabell, C. A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2000-02-07
- Volume
- 148
- Issue
- 3
- Pages
- 519-530
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2099573412
- Cited by
- 447
- References
- 31
- FWCI
- 15.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Taunton, J., Rowning, B. A., Coughlin, M. L., Wu, M., Moon, R. T., Mitchison, T. J., & Larabell, C. A. (2000). Actin-Dependent Propulsion of Endosomes and Lysosomes by Recruitment of N-Wasp✪. The Journal of Cell Biology. https://doi.org/10.1083/jcb.148.3.519
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