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Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent
Open accessAbstract
Previous results, based on inhibition of fertilization by an anti–α6 integrin mAb (GoH3), suggest that the α6β1 integrin on mouse eggs functions as the receptor for sperm (Almeida, E.A., A.P. Huovila, A.E. Sutherland, L.E. Stephens, P.G. Calarco, L.M. Shaw, A.M. Mercurio, A. Sonnenberg, P. Primakoff, D.G. Myles, and J.M. White. 1995. Cell. 81:1095–1104). Because the egg surface tetraspanin CD9 is essential for gamete fusion (Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Suzuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324) and CD9 is known to associate with integrins, recent models of gamete fusion have posited that egg CD9 acts in association with α6β1 in fusion (Chen, M.S., K.S. Tung, S.A. Coonrod, Y. Takahashi, D. Bigler, A. Chang, Y. Yamashita, P.W. Kincade, J.C. Herr, and J.M. White. 1999. Proc. Natl. Acad. Sci. USA. 96:11830–11835; Kaji, K., S. Oda, T. Shikano, T. Ohnuki, Y. Uematsu, J. Sakagami, N. Tada, S. Miyazaki, and A. Kudo. 2000. Nat. Genet. 24:279–282; Le Naour, F., E. Rubinstein, C. Jasmin, M. Prenant, and C. Boucheix. 2000. Science. 287:319–321; Miyado, K., G. Yamada, S. Yamada, H. Hasuwa, Y. Nakamura, F. Ryu, K. Su- zuki, K. Kosai, K. Inoue, A. Ogura, M. Okabe, and E. Mekada. 2000. Science. 287:321–324). Using eggs from cultured ovaries of mice lacking the α6 integrin subunit, we found that the fertilization rate, fertilization index, and sperm binding were not impaired compared with wild-type or heterozygous controls. Furthermore, a reexamination of antibody inhibition, using an assay that better simulates in vivo fertilization conditions, revealed no inhibition of fusion by the GoH3 mAb. We also found that an anti-CD9 mAb completely blocks sperm fusion with either wild-type eggs or eggs lacking α6β1. Based on these results, we conclude that the α6β1 integrin is not essential for sperm–egg fusion, and we suggest a new model in which CD9 acts by itself, or interacts with egg protein(s) other than α6β1, to function in sperm–egg fusion.
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Citations
Cited by
Hemler, M. E. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111301.153609 · Cited by 831
Calcium at Fertilization and in Early Development
Whitaker, M. (2006) · Physiological Reviews · doi:10.1152/physrev.00023.2005 · Cited by 512
A profile of fertilization in mammals
Wassarman, P. M., Jovine, L., & Litscher, E. S. (2001) · Nature Cell Biology · doi:10.1038/35055178 · Cited by 393
Egg Activation at Fertilization: Where It All Begins
Runft, L. L., Jaffe, L. A., & Mehlmann, L. M. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0600 · Cited by 364
New insights into the molecular mechanisms of sperm-egg interaction
Nixon, B., Aitken, R. J., & McLaughlin, E. A. (2007) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-6552-x · Cited by 103
Metadata
- Authors
- Miller, B. J., Georges-Labouesse, E., Primakoff, P., & Myles, D. G.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2000-06-12
- Volume
- 149
- Issue
- 6
- Pages
- 1289-1296
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2336731832
- Cited by
- 177
- References
- 0
- FWCI
- 8.55
- Type
- journal article
- Language
- en
- Open access
- green
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- Miller, B. J., Georges-Labouesse, E., Primakoff, P., & Myles, D. G. (2000). Normal Fertilization Occurs with Eggs Lacking the Integrin α6β1 and Is Cd9-Dependent. The Journal of Cell Biology. https://doi.org/10.1083/jcb.149.6.1289
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