The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Restricted Accumulation of Phosphatidylinositol 3-Kinase Products in a Plasmalemmal Subdomain during Fcγ Receptor-Mediated Phagocytosis
Open accessAbstract
Phagocytosis is a highly localized and rapid event, requiring the generation of spatially and temporally restricted signals. Because phosphatidylinositol 3-kinase (PI3K) plays an important role in the innate immune response, we studied the generation and distribution of 3′ phosphoinositides (3′PIs) in macrophages during the course of phagocytosis. The presence of 3′PI was monitored noninvasively in cells transfected with chimeras of green fluorescent protein and the pleckstrin homology domain of either Akt, Btk, or Gab1. Although virtually undetectable in unstimulated cells, 3′PI rapidly accumulated at sites of phagocytosis. This accumulation was sharply restricted to the phagosomal cup, with little 3′PI detectable in the immediately adjacent areas of the plasmalemma. Measurements of fluorescence recovery after photobleaching were made to estimate the mobility of lipids in the cytosolic monolayer of the phagosomal membrane. Stimulation of phagocytic receptors induced a marked reduction of lipid mobility that likely contributes to the restricted distribution of 3′PI at the cup. 3′PI accumulation during phagocytosis was transient, terminating shortly after sealing of the phagosomal vacuole. Two factors contribute to the rapid disappearance of 3′PI: the dissociation of the type I PI3K from the phagosomal membrane and the persistent accumulation of phosphoinositide phosphatases.
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Cites
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BackgroundProtein Kinase C as a Molecular Machine for Decoding Calcium and Diacylglycerol Signals
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Self-citationEssential Role for Cholesterol in Entry of Mycobacteria into Macrophages
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Localized Biphasic Changes in Phosphatidylinositol-4,5-Bisphosphate at Sites of Phagocytosis
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“As reported previously (Botelho et al., 2000), PI(4,5)P 2 undergoes a biphasic change: a small transient increase followed immediately by its virtual disappearance from the phagosomal membrane.”
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Cited by
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation
Balla, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00028.2012 · Cited by 1,638
Self-citationPIP2and Proteins: Interactions, Organization, and Information Flow
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Phagocytosis: Our Current Understanding of a Universal Biological Process
Uribe-Querol, E., & Rosales, C. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.01066 · Cited by 651
Continuous T cell receptor signaling required for synapse maintenance and full effector potential
Huppa, J. B., Gleimer, M., Sumen, C., & Davis, M. M. (2003) · Nature Immunology · doi:10.1038/ni951 · Cited by 401
Cdc42, Rac1, and Rac2 Display Distinct Patterns of Activation during Phagocytosis
Hoppe, A. D., & Swanson, J. A. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-11-0847 · Cited by 365
Metadata
- Authors
- Marshall, J. G., Booth, J. W., Stambolic, V., Mak, T., Balla, T., Schreiber, A. D., Meyer, T., & Grinstein, S.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-06-18
- Volume
- 153
- Issue
- 7
- Pages
- 1369-1380
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2145355599
- Cited by
- 281
- References
- 10
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Marshall, J. G., Booth, J. W., Stambolic, V., Mak, T., Balla, T., Schreiber, A. D., Meyer, T., & Grinstein, S. (2001). Restricted Accumulation of Phosphatidylinositol 3-Kinase Products in a Plasmalemmal Subdomain during Fcγ Receptor-Mediated Phagocytosis. The Journal of Cell Biology. https://doi.org/10.1083/jcb.153.7.1369
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