Essential Role for Cholesterol in Entry of Mycobacteria into Macrophages
Open accessAbstract
Mycobacteria are intracellular pathogens that can invade and survive within host macrophages, thereby creating a major health problem worldwide. The molecular mechanisms involved in mycobacterial entry are still poorly characterized. Here we report that cholesterol is essential for uptake of mycobacteria by macrophages. Cholesterol accumulated at the site of mycobacterial entry, and depleting plasma membrane cholesterol specifically inhibited mycobacterial uptake. Cholesterol also mediated the phagosomal association of TACO, a coat protein that prevents degradation of mycobacteria in lysosomes. Thus, by entering host cells at cholesterol-rich domains of the plasma membrane, mycobacteria may ensure their subsequent intracellular survival in TACO-coated phagosomes.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Cholesterol rich membrane rafts have been shown to mediate phagocytosis of Mycobacterium, Pseudomonas or Leishmania species in macrophages.
“Cholesterol rich membrane rafts have been shown to mediate phagocytosis of Mycobacterium, Pseudomonas or Leishmania species in macrophages [41-44].”
From Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium (2008) · cites this paper as [41]
Cites
Functional rafts in cell membranes
Simons, K., & Ikonen, E. (1997) · Nature · doi:10.1038/42408 · Cited by 9,757 · see citing works
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Cole, S. T., Brosch, R., Parkhill, J., Garnier, T., Churcher, C., Harris, D., Gordon, S. V., Eiglmeier, K., Gas, S., Barry, C. E., et al. (1998) · Nature · doi:10.1038/31159 · Cited by 7,847 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death
Sborgi, L., Rühl, S., Mulvihill, E., Pipercevic, J., Heilig, R., Stahlberg, H., Farady, C. J., Müller, D. J., Broz, P., & Hiller, S. (2016) · The EMBO Journal · doi:10.15252/embj.201694696 · Cited by 1,152 · see citing works
<i>Mycobacterium tuberculosis</i>Pathogenesis and Molecular Determinants of Virulence
Smith, I. (2003) · Clinical Microbiology Reviews · doi:10.1128/cmr.16.3.463-496.2003 · Cited by 1,154 · see citing works
Mycobacterial persistence requires the utilization of host cholesterol
Pandey, A. K., & Sassetti, C. M. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0711159105 · Cited by 1,064 · see citing works
Protein Kinase G from Pathogenic Mycobacteria Promotes Survival Within Macrophages
Walburger, A., Koul, A., Ferrari, G., Nguyen, L., Prescianotto-Baschong, C., Huygen, K., Klebl, B., Thompson, C., Bacher, G., & Pieters, J. (2004) · Science · doi:10.1126/science.1099384 · Cited by 595 · see citing works
Self-citationSurvival of Mycobacteria in Macrophages Is Mediated by Coronin 1-Dependent Activation of Calcineurin
Jayachandran, R., Sundaramurthy, V., Combaluzier, B., Mueller, P., Korf, H., Huygen, K., Miyazaki, T., Albrecht, I., Massner, J., & Pieters, J. (2007) · Cell · doi:10.1016/j.cell.2007.04.043 · Cited by 310 · see citing works
Self-citationD’Avila, H., Melo, R. C. N., Parreira, G. G., Werneck-Barroso, E., Castro-Faria-Neto, H. C., & Bozza, P. T. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.5.3087 · Cited by 289 · see citing works
Lafont, F., Van Nhieu, G. T., Hanada, K., Sansonetti, P., & van der Goot, F. G. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf457 · Cited by 242 · see citing works
Jong, A., Wu, C.-H., Shackleford, G. M., Kwon-Chung, K. J., Chang, Y. C., Chen, H.-M., Ouyang, Y., & Huang, S.-H. (2008) · Cellular Microbiology · doi:10.1111/j.1462-5822.2008.01128.x · Cited by 112 · see citing works
Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium
Sillo, A., Bloomfield, G., Balest, A., Balbo, A., Pergolizzi, B., Peracino, B., Skelton, J., Ivens, A., & Bozzaro, S. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-291 · Cited by 69 · see citing works
Dominguez, J., Mendes, A. I., Pacheco, A. R., Peixoto, M. J., Pedrosa, J., & Fraga, A. G. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1266261 · Cited by 4 · see citing works
- Authors
- Gatfield, J., & Pieters, J.
- Affiliations
- Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland.
- Corresponding author
- Pieters, Jean
- Venue
- Science
- Published
- 2000-06-02
- Volume
- 288
- Issue
- 5471
- Pages
- 1647-1651
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2012416622
- Cited by
- 615
- References
- 2
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 26 · 2023: 13 · 2022: 29 · 2021: 10 · 2020: 19 · 2019: 21 · 2018: 25 · 2017: 20 · 2016: 31 · 2015: 22 · 2014: 30 · 2013: 21 · 2012: 28
- FWCI
- 25.39
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- edoc (University of Basel) (acceptedVersion)
- edoc (University of Basel)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- mycobacteria, macrophages, cholesterol, bacterial internalization, plasma membrane cholesterol, phagosome, tacos, phagosome-lysosome fusion, intracellular survival, host-pathogen interaction
- MeSH terms
- Animals, Cell Line, Cell Membrane, Cell Movement, Cholesterol, Macrophages, Membrane Fluidity, Mice, Inbred C57BL, Microfilament Proteins, Mycobacterium bovis, Phagocytosis, Phagosomes, Proteins, Protein Biosynthesis, Macrophage-1 Antigen, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
