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Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics
Open accessAbstract
Tropomyosin binds to actin filaments and is implicated in stabilization of actin cytoskeleton. We examined biochemical and cell biological properties of Caenorhabditis elegans tropomyosin (CeTM) and obtained evidence that CeTM is antagonistic to ADF/cofilin-dependent actin filament dynamics. We purified CeTM, actin, and UNC-60B (a muscle-specific ADF/cofilin isoform), all of which are derived from C. elegans, and showed that CeTM and UNC-60B bound to F-actin in a mutually exclusive manner. CeTM inhibited UNC-60B–induced actin depolymerization and enhancement of actin polymerization. Within isolated native thin filaments, actin and CeTM were detected as major components, whereas UNC-60B was present at a trace amount. Purified UNC-60B was unable to interact with the native thin filaments unless CeTM and other associated proteins were removed by high-salt extraction. Purified CeTM was sufficient to restore the resistance of the salt-extracted filaments from UNC-60B. In muscle cells, CeTM and UNC-60B were localized in different patterns. Suppression of CeTM by RNA interference resulted in disorganized actin filaments and paralyzed worms in wild-type background. However, in an ADF/cofilin mutant background, suppression of CeTM did not worsen actin organization and worm motility. These results suggest that tropomyosin is a physiological inhibitor of ADF/cofilin-dependent actin dynamics.
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Cites
Control of actin dynamics in cell motility
Carlier, M.-F., & Pantaloni, D. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1997.1062 · Cited by 351
Cited by
Actin stress fibers – assembly, dynamics and biological roles
Tojkander, S., Gateva, G., & Lappalainen, P. (2012) · Journal of Cell Science · doi:10.1242/jcs.098087 · Cited by 900
Tropomyosin-Based Regulation of the Actin Cytoskeleton in Time and Space
Gunning, P., O’neill, G., & Hardeman, E. (2008) · Physiological Reviews · doi:10.1152/physrev.00001.2007 · Cited by 478
Actin dynamics associated with focal adhesions
Ciobanasu, C., Faivre, B., & Le Clainche, C. (2012) · International Journal of Cell Biology · doi:10.1155/2012/941292 · Cited by 63
Anyanful, A., Ono, K., Johnsen, R. C., Ly, H., Jensen, V., Baillie, D. L., & Ono, S. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200407085 · Cited by 58
“Western blot was performed as described previously (Ono and Ono, 2002) using the following primary antibodies: anti–UNC-60A (Ono et al.”
MethodSelf-citationStructural basis underlying specific biochemical activities of non-muscle tropomyosin isoforms
Selvaraj, M., Kokate, S. B., Reggiano, G., Kogan, K., Kotila, T., Kremneva, E., DiMaio, F., Lappalainen, P., & Huiskonen, J. T. (2023) · Cell Reports · doi:10.1016/j.celrep.2022.111900 · Cited by 22
Metadata
- Authors
- Ono, S., & Ono, K.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2002-03-18
- Volume
- 156
- Issue
- 6
- Pages
- 1065-1076
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2140686956
- Cited by
- 256
- References
- 1
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- green
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