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Cited by 63Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchSkin and Cellular Biology Research

Actin dynamics associated with focal adhesions

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Ciobanasu, Corina, Faivre, Bruno, Le Clainche, Christophe·2012·International Journal of Cell Biology
DOI 10.1155/2012/941292PMC PMC3312244
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Abstract

Cell-matrix adhesion plays a major role during cell migration. Proteins from adhesion structures connect the extracellular matrix to the actin cytoskeleton, allowing the growing actin network to push the plasma membrane and the contractile cables (stress fibers) to pull the cell body. Force transmission to the extracellular matrix depends on several parameters including the regulation of actin dynamics in adhesion structures, the contractility of stress fibers, and the mechanosensitive response of adhesion structures. Here we highlight recent findings on the molecular mechanisms by which actin assembly is regulated in adhesion structures and the molecular basis of the mechanosensitivity of focal adhesions.

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  • Contractile actin filament assembly, stress fiber assembly, and regulation of actin filament-based processes are molecular processes centrally involved in focal adhesion biology.

    “all of which are molecular processes centrally involved in focal adhesion biology (Burridge and Guilluy, 2016; Ciobanasu et al., 2012; Geiger et al., 2009; Wozniak et al., 2004).”

    From Alzheimer’s genetic risk factor CASS4 controls microglial focal adhesion biology and amyloidosis (2026) · cites this paper as Ciobanasu et al., 2012

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  1. [1]Wang W Goswami S Sahai E Wyckoff JB Segall JE Condeelis JS Tumor cells caught in the act of invading: their strategy for enhanced cell motility Trends in Cell Biology 2005 15 3 138 145 15752977
  2. [2]Ridley AJ Schwartz MA Burridge K Cell migration: integrating signals from front to back Science 2003 302 5651 1704 1709 14657486
  3. [3]Le Clainche C Carlier MF Regulation of actin assembly associated with protrusion and adhesion in cell migration Physiological Reviews 2008 88 2 489 513 18391171
  4. [4]Critchley DR Biochemical and structural properties of the integrin-associated cytoskeletal protein talin Annual Review of Biophysics 2009 38 1 235 254 19416068
  5. [5]Zaidel-Bar R Ballestrem C Kam Z Geiger B Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells Journal of Cell Science 2003 116, part 22 4605 4613 14576354
  6. [6]Jiang G Giannone G Critchley DR Fukumoto E Sheet MP Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin Nature 2003 424 6946 334 337 12867986
  7. [7]Tadokoro S Shattil SJ Eto K Talin binding to integrin β tails: a final common step in integrin activation Science 2003 302 5642 103 106 14526080
  8. [8]Choi CK Vicente-Manzanares M Zareno J Whitmore LA Mogilner A Horwitz AR Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner Nature Cell Biology 2008 10 9 1039 1050 19160484 PMC2827253
  9. [9]Otey CA Pavalko FM Burridge K An interaction between α-actinin and the β1 integrin subunit in vitro Journal of Cell Biology 1990 111 2 721 729 2116421 PMC2116186
  10. [10]Kanchanawong P Shtengel G Pasapera AM Nanoscale architecture of integrin-based cell adhesions Nature 2010 468 7323 580 584 21107430 PMC3046339
  11. [11]Nemethova M Auinger S Small JV Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella Journal of Cell Biology 2008 180 6 1233 1244 18362182 PMC2290848
  12. [12]Gardel ML Schneider IC Aratyn-Schaus Y Waterman CM Mechanical integration of actin and adhesion dynamics in cell migration Annual Review of Cell and Developmental Biology 2010 26 315 333 PMC4437624 19575647
  13. [13]Chrzanowska-Wodnicka M Burridge K Rho-stimulated contractility drives the formation of stress fibers and focal adhesions Journal of Cell Biology 1996 133 6 1403 1415 8682874 PMC2120895
  14. [14]Small JV Rottner K Kaverina I Anderson KI Assembling an actin cytoskeleton for cell attachment and movement Biochimica et Biophysica Acta 1998 1404 3 271 281 9739149
  15. [15]Geiger B Spatz JP Bershadsky AD Environmental sensing through focal adhesions Nature Reviews Molecular Cell Biology 2009 10 1 21 33 19197329
  16. [16]Riveline D Zamir E Balaban NQ Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism Journal of Cell Biology 2001 153 6 1175 1186 11402062 PMC2192034
  17. [17]Drees BE Andrews KM Beckerle MC Molecular dissection of zyxin function reveals its involvement in cell motility Journal of Cell Biology 1999 147 7 1549 1560 10613911 PMC2174240
  18. [18]Kuo JC Han X Hsiao CT Iii JRY Waterman CM Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation Nature Cell Biology 2011 13 4 383 393 21423176 PMC3279191
  19. [19]Johnson CP Tang HY Carag C Speicher DW Discher DE Forced unfolding of proteins within cells Science 2007 317 5838 663 666 17673662 PMC2741095
  20. [20]Grashoff C Hoffman BD Brenner MD Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics Nature 2010 466 7303 263 266 20613844 PMC2901888
  21. [21]del Rio A Perez-Jimenez R Liu R Roca-Cusachs P Fernandez JM Sheetz MP Stretching single talin rod molecules activates vinculin binding Science 2009 323 5914 638 641 19179532 PMC9339221
  22. [22]Pasapera AM Schneider IC Rericha E Schlaepfer DD Waterman CM Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation Journal of Cell Biology 2010 188 6 877 890 20308429 PMC2845065
  23. [23]Liu J Taylor DW Taylor KA A 3-D reconstruction of smooth muscle α-actinin by CryoEm reveals two different conformations at the actin-binding region Journal of Molecular Biology 2004 338 1 115 125 15050827
  24. [24]Langanger G Moeremans M Daneels G The molecular organization of myosin in stress fibers of cultured cells Journal of Cell Biology 1986 102 1 200 209 3510218 PMC2114045
  25. [25]Taylor KA Taylor DW Schachat F Isoforms of α-actinin from cardiac, smooth, and skeletal muscle form polar arrays of actin filaments Journal of Cell Biology 2000 149 3 635 645 10791977 PMC2174853
  26. [26]Vicente-Manzanares M Ma X Adelstein RS Horwitz AR Non-muscle myosin II takes centre stage in cell adhesion and migration Nature Reviews Molecular Cell Biology 2009 10 11 778 790 19851336 PMC2834236
  27. [27]Verkhovsky AB Svitkina TM Borisy GG Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles Journal of Cell Biology 1995 131 4 989 1002 7490299 PMC2200006
  28. [29]Gupton SL Anderson KL Kole TP Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin Journal of Cell Biology 2005 168 4 619 631 15716379 PMC2171771
  29. [30]Ono S Ono K Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics Journal of Cell Biology 2002 156 6 1065 1076 11901171 PMC2173459
  30. [31]Bugyi B Didry D Carlier MF How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach EMBO journal 2010 29 1 14 26 19893490 PMC2808365
  31. [32]Blanchoin L Pollard TD Hitchcock-DeGregori SE Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin Current Biology 2001 11 16 1300 1304 11525747
  32. [33]Tojkander S Gateva G Schevzov G A molecular pathway for myosin II recruitment to stress fibers Current Biology 2011 21 7 539 550 21458264
  33. [34]Hotulainen P Lappalainen P Stress fibers are generated by two distinct actin assembly mechanisms in motile cells Journal of Cell Biology 2006 173 3 383 394 16651381 PMC2063839
  34. [35]Pellegrin S Mellor H Actin stress fibers Journal of Cell Science 2007 120, part 20 3491 3499 17928305
  35. [36]Naumanen P Lappalainen P Hotulainen P Mechanisms of actin stress fibre assembly Journal of Microscopy 2008 231 3 446 454 18755000
  36. [37]Zhang XF Schaefer AW Burnette DT Schoonderwoert VT Forscher P Rho-dependent contractile responses in the neuronal growth cone are independent of classical peripheral retrograde actin flow Neuron 2003 40 5 931 944 14659092
  37. [38]Yoshigi M Hoffman LM Jensen CC Yost HJ Beckerle MC Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement Journal of Cell Biology 2005 171 2 209 215 16247023 PMC2171187
  38. [39]Smith MA Blankman E Gardel ML Luettjohann L Waterman CM Beckerle MC A zyxin-mediated mechanism for actin stress fiber maintenance and repair Developmental Cell 2010 19 3 365 376 20833360 PMC2954498
  39. [40]Hu K Ji L Applegate KT Danuser G Waterman-Storer CM Differential transmission of actin motion within focal adhesions Science 2007 315 5808 111 115 17204653
  40. [41]Le Clainche C Dwivedi SP Didry D Carlier MF Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein Journal of Biological Chemistry 2010 285 30 23420 23432 20484056 PMC2906333
  41. [42]Breitsprecher D Kiesewetter AK Linkner J Molecular mechanism of Ena/VASP-mediated actin-filament elongation EMBO Journal 2011 30 3 456 467 21217643 PMC3034019
  42. [43]Breitsprecher D Kiesewetter AK Linkner J Clustering of VASP actively drives processive, WH2 domain-mediated actin filament elongation EMBO Journal 2008 27 22 2943 2954 18923426 PMC2585163
  43. [44]Reinhard M Halbrugge M Scheer U Wiegand C Jockusch BM Walter U The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts EMBO Journal 1992 11 6 2063 2070 1318192 PMC556672
  44. [45]Rottner K Behrendt B Small JV Wehland J VASP dynamics during lamellipodia protrusion Nature Cell Biology 1999 1 5 321 322 10559946
  45. [46]Chereau D Dominguez R Understanding the role of the G-actin-binding domain of Ena/VASP in actin assembly Journal of Structural Biology 2006 155 2 195 201 16684607
  46. [47]Schirenbeck A Arasada R Bretschneider T Stradal TEB Schleicher M Faix J The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation Proceedings of the National Academy of Sciences of the United States of America 2006 103 20 7694 7699 16675552 PMC1457090
  47. [48]Laurent V Loisel TP Harbeck B Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes Journal of Cell Biology 1999 144 6 1245 1258 10087267 PMC2150578
  48. [49]Castellano F Le Clainche C Patin D Carlier MF Chavrier P A WASp-VASP complex regulates actin polymerization at the plasma membrane EMBO Journal 2001 20 20 5603 5614 11598004 PMC125672
  49. [50]Samarin S Romero S Kocks C Didry D Pantaloni D Carlier MF How VASP enhances actin-based motility Journal of Cell Biology 2003 163 1 131 142 14557252 PMC2173428
  50. [51]Bear JE Svitkina TM Krause M Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility Cell 2002 109 4 509 521 12086607
  51. [52]Hansen SD Mullins RD VASP is a processive actin polymerase that requires monomeric actin for barbed end association Journal of Cell Biology 2010 191 3 571 584 21041447 PMC3003327
  52. [53]Bear JE Loureiro JJ Libova I Fässler R Wehland J Gertler FB Negative regulation of fibroblast motility by Ena/VASP proteins Cell 2000 101 7 717 728 10892743
  53. [54]Fradelizi J Noireaux V Plastino J ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity Nature Cell Biology 2001 3 8 699 707 11483954
  54. [55]Xu W Coll JL Adamson ED Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells; effects on cell locomotion by domain deleted vinculin Journal of Cell Science 1998 111, part 11 1535 1544 9580561
  55. [56]Saunders RM Holt MR Jennings L Role of vinculin in regulating focal adhesion turnover European Journal of Cell Biology 2006 85 6 487 500 16584805
  56. [57]Izard T Evans G Borgon RA Rush CL Bricogne G Bois PRJ Vinculin activation by talin through helical bundle conversion Nature 2004 427 6970 171 175 14702644
  57. [58]Bourdet-Sicard R Rüdiger M Jockusch BM Gounon P Sansonetti PJ van Nhieu GT Binding of the Shigella protein IpaA to vinculin induces F-actin depolymerization EMBO Journal 1999 18 21 5853 5862 10545097 PMC1171651
  58. [59]Ramarao N Le Clainche C Izard T Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain FEBS Letters 2007 581 5 853 857 17289036
  59. [60]Wen KK Rubenstein PA DeMali KA Vinculin nucleates actin polymerization and modifies actin filament structure Journal of Biological Chemistry 2009 284 44 30463 30473 19736312 PMC2781601
  60. [61]Goode BL Eck MJ Mechanism and function of formins in the control of actin assembly Annual Review of Biochemistry 2007 76 593 627 17373907
  61. [62]Kovar DR Harris ES Mahaffy R Higgs HN Pollard TD Control of the assembly of ATP-and ADP-actin by formins and profilin Cell 2006 124 2 423 435 16439214
  62. [63]Romero S Le Clainche C Didry D Egile C Pantaloni D Carlier MF Formin is a processive motor that requires profilin to accelerate actin assembly and associated ATP hydrolysis Cell 2004 119 3 419 429 15507212
  63. [64]Watanabe N Kato T Fujita A Ishizaki T Narumiya S Cooperation between mDia1 and ROCK in Rho-induced actin reorganization Nature Cell Biology 1999 1 3 136 143 10559899
  64. [65]Gupton SL Eisenmann K Alberts AS Waterman-Storer CM mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration Journal of Cell Science 2007 120, part 19 3475 3487 17855386

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Authors
Ciobanasu, C., Faivre, B., & Le Clainche, C.
Affiliations
  • Laboratoire d'Enzymologie et Biochimie Structurales, Centre de Recherche CNRS de Gif-sur-Yvette, avenue de la Terrasse, 91198 Gif-sur-Yvette, France
  • Centre National de la Recherche Scientifique (FR)
ORCID
Faivre, Bruno
Venue
International Journal of Cell Biology
Publisher
Wiley
Published
2012
Volume
2012
Pages
1-9
ISSN
  • 1687-8876 (ISSN-L)
  • 1687-8884
DOI
10.1155/2012/941292
PMC
PMC3312244
OpenAlex
W2049430206
Cited by
63
References
1
Citations by year
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FWCI
2.12
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Cell Biology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Agence Nationale de la Recherche (ANR-09-JCJC-0111)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
Keywords
focal adhesions, actin dynamics, cell-matrix adhesion, cell migration, stress fibers, mechanosensitivity, extracellular matrix, contractility, force transmission

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