The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Apaf-1 and caspase-9 accelerate apoptosis, but do not determine whether factor-deprived or drug-treated cells die
Open accessAbstract
Apoptosis after growth factor withdrawal or drug treatment is associated with mitochondrial cytochrome c release and activation of Apaf-1 and caspase-9. To determine whether loss of Apaf-1, caspase-2, and caspase-9 prevented death of factor-starved cells, allowing them to proliferate when growth factor was returned, we generated IL-3–dependent myeloid lines from gene-deleted mice. Long after growth factor removal, cells lacking Apaf-1, caspase-9 or both caspase-9 and caspase-2 appeared healthy, retained intact plasma membranes, and did not expose phosphatidylserine. However, release of cytochrome c still occurred, and they failed to form clones when IL-3 was restored. Cells lacking caspase-2 alone had no survival advantage. Therefore, Apaf-1, caspase-2, and caspase-9 are not required for programmed cell death of factor-dependent cells, but merely affect its rate. In contrast, transfection with Bcl-2 provided long-term, clonogenic protection, and could act independently of the apoptosome. Unlike expression of Bcl-2, loss of Apaf-1, caspase-2, or caspase-9 would therefore be unlikely to enhance the survival of cancer cells.
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Marsden, V. S., O'Connor, L., O'Reilly, L. A., Silke, J., Metcalf, D., Ekert, P. G., Huang, D. C. S., Cecconi, F., Kuida, K., Tomaselli, K. J., et al. (2002) · Nature · doi:10.1038/nature01101 · Cited by 578
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“In contrast, Apaf-1- or caspase-deficient cells exhibit only short-term resistance to apoptotic stimuli and do not retain clonogenic potential (Ekert et al., 2004; Marsden et al., 2002; van Delft et al., 2010).”
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Metadata
- Authors
- Ekert, P. G., Read, S. H., Silke, J., Marsden, V. S., Kaufmann, H., Hawkins, C. J., Gerl, R., Kumar, S., & Vaux, D. L.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2004-06-21
- Volume
- 165
- Issue
- 6
- Pages
- 835-842
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2170848670
- Cited by
- 181
- References
- 1
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Ekert, P. G., Read, S. H., Silke, J., Marsden, V. S., Kaufmann, H., Hawkins, C. J., Gerl, R., Kumar, S., & Vaux, D. L. (2004). Apaf-1 and caspase-9 accelerate apoptosis, but do not determine whether factor-deprived or drug-treated cells die. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200312031
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