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Cell migration without a lamellipodium
Open accessAbstract
The actin cytoskeleton is locally regulated for functional specializations for cell motility. Using quantitative fluorescent speckle microscopy (qFSM) of migrating epithelial cells, we previously defined two distinct F-actin networks based on their F-actin–binding proteins and distinct patterns of F-actin turnover and movement. The lamellipodium consists of a treadmilling F-actin array with rapid polymerization-dependent retrograde flow and contains high concentrations of Arp2/3 and ADF/cofilin, whereas the lamella exhibits spatially random punctae of F-actin assembly and disassembly with slow myosin-mediated retrograde flow and contains myosin II and tropomyosin (TM). In this paper, we microinjected skeletal muscle αTM into epithelial cells, and using qFSM, electron microscopy, and immunolocalization show that this inhibits functional lamellipodium formation. Cells with inhibited lamellipodia exhibit persistent leading edge protrusion and rapid cell migration. Inhibition of endogenous long TM isoforms alters protrusion persistence. Thus, cells can migrate with inhibited lamellipodia, and we suggest that TM is a major regulator of F-actin functional specialization in migrating cells.
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Citations
Cited by
Actin and Actin-Binding Proteins
Pollard, T. D. (2016) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a018226 · Cited by 912
Actin stress fibers – assembly, dynamics and biological roles
Tojkander, S., Gateva, G., & Lappalainen, P. (2012) · Journal of Cell Science · doi:10.1242/jcs.098087 · Cited by 900
Spatiotemporal Feedback between Actomyosin and Focal-Adhesion Systems Optimizes Rapid Cell Migration
Gupton, S. L., & Waterman-Storer, C. M. (2006) · Cell · doi:10.1016/j.cell.2006.05.029 · Cited by 631
Self-citationTropomyosin-Based Regulation of the Actin Cytoskeleton in Time and Space
Gunning, P., O’neill, G., & Hardeman, E. (2008) · Physiological Reviews · doi:10.1152/physrev.00001.2007 · Cited by 478
Alexandrova, A. Y., Arnold, K., Schaub, S., Vasiliev, J. M., Meister, J.-J., Bershadsky, A. D., & Verkhovsky, A. B. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003234 · Cited by 275
Metadata
- Authors
- Gupton, S. L., Anderson, K. L., Kole, T. P., Fischer, R. S., Ponti, A., Hitchcock-DeGregori, S. E., Danuser, G., Fowler, V. M., Wirtz, D., Hanein, D., & Waterman-Storer, C. M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2005-02-14
- Volume
- 168
- Issue
- 4
- Pages
- 619-631
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2144786034
- Cited by
- 266
- References
- 0
- FWCI
- 11.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Gupton, S. L., Anderson, K. L., Kole, T. P., Fischer, R. S., Ponti, A., Hitchcock-DeGregori, S. E., Danuser, G., Fowler, V. M., Wirtz, D., Hanein, D., & Waterman-Storer, C. M. (2005). Cell migration without a lamellipodium. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200406063
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