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Cited by 312Cellular transport and secretionFungal and yeast genetics researchExtracellular vesicles in disease

Vesicles carry most exocyst subunits to exocytic sites marked by the remaining two subunits, Sec3p and Exo70p

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Boyd, Charles, Hughes, Thom, Pypaert, Marc, Novick, Peter·2004·The Journal of Cell Biology
DOI 10.1083/jcb.200408124PMC PMC2172445
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Abstract

Exocytosis in the budding yeast Saccharomyces cerevisiae occurs at discrete domains of the plasma membrane. The protein complex that tethers incoming vesicles to sites of secretion is known as the exocyst. We have used photobleaching recovery experiments to characterize the dynamic behavior of the eight subunits that make up the exocyst. One subset (Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, and Exo84p) exhibits mobility similar to that of the vesicle-bound Rab family protein Sec4p, whereas Sec3p and Exo70p exhibit substantially more stability. Disruption of actin assembly abolishes the ability of the first subset of subunits to recover after photobleaching, whereas Sec3p and Exo70p are resistant. Immunogold electron microscopy and epifluorescence video microscopy indicate that all exocyst subunits, except for Sec3p, are associated with secretory vesicles as they arrive at exocytic sites. Assembly of the exocyst occurs when the first subset of subunits, delivered on vesicles, joins Sec3p and Exo70p on the plasma membrane. Exocyst assembly serves to both target and tether vesicles to sites of exocytosis.

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  • The distribution of the exocyst within a cell can be highly dynamic, consistent with its role in directing membrane fusion to specific domains.

    “The distribution of the exocyst within a cell can be highly dynamic, consistent with its role in directing membrane fusion to specific domains (Boyd et al, 2004;Beronja et al, 2005;Mehta et al, 2005;Zhang et al, 2008;Murthy et al, 2010).”

    From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Boyd et al, 2004

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Cited by

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Authors
Boyd, C., Hughes, T., Pypaert, M., & Novick, P.
Affiliations
  • Yale University (US)
  • 2Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT 59717
Corresponding author
Novick, Peter
ORCID
Novick, Peter
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2004-12-06
Volume
167
Issue
5
Pages
889-901
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.200408124
PMC
PMC2172445
OpenAlex
W2106457287
Cited by
312
References
1
Citations by year
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FWCI
4.72
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • National Cancer Institute (P01CA046128, CA46128)
  • National Institute of General Medical Sciences (R37GM035370, GM 35370, R01GM035370)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK0717-28)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
exocyst, exocytosis, vesicle tethering, secretory vesicles, FRAP, actin assembly, plasma membrane, Saccharomyces cerevisiae
MeSH terms
Cell Membrane, Exocytosis, Immunohistochemistry, Membrane Fusion, Actin Cytoskeleton, Saccharomyces cerevisiae, Protein Transport, Cell Surface Extensions, Secretory Vesicles, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, Fluorescence Recovery After Photobleaching, Microscopy, Electron, Transmission, Macromolecular Substances

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