The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin-II heavy chain
Open accessAbstract
On rigid surfaces, the cytoskeleton of migrating cells is polarized, but tissue matrix is normally soft. We show that nonmuscle MIIB (myosin-IIB) is unpolarized in cells on soft matrix in 2D and also within soft 3D collagen, with rearward polarization of MIIB emerging only as cells migrate from soft to stiff matrix. Durotaxis is the tendency of cells to crawl from soft to stiff matrix, and durotaxis of primary mesenchymal stem cells (MSCs) proved more sensitive to MIIB than to the more abundant and persistently unpolarized nonmuscle MIIA (myosin-IIA). However, MIIA has a key upstream role: in cells on soft matrix, MIIA appeared diffuse and mobile, whereas on stiff matrix, MIIA was strongly assembled in oriented stress fibers that MIIB then polarized. The difference was caused in part by elevated phospho-S1943–MIIA in MSCs on soft matrix, with site-specific mutants revealing the importance of phosphomoderated assembly of MIIA. Polarization is thus shown to be a highly regulated compass for mechanosensitive migration.
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Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Self-citationTissue Cells Feel and Respond to the Stiffness of Their Substrate
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Self-citationBone marrow cells regenerate infarcted myocardium
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Cell locomotion and focal adhesions are regulated by substrate flexibility
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Mesenchymal Stromal Cells: Current Understanding and Clinical Status
Salem, H. K., & Thiemermann, C. (2010) · Stem Cells · doi:10.1002/stem.269 · Cited by 915
One-dimensional topography underlies three-dimensional fibrillar cell migration
Doyle, A. D., Wang, F. W., Matsumoto, K., & Yamada, K. M. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200810041 · Cited by 732
Mesenchymal stem cell injection after myocardial infarction improves myocardial compliance
Berry, M. F., Engler, A. J., Woo, Y. J., Pirolli, T. J., Bish, L. T., Jayasankar, V., Morine, K. J., Gardner, T. J., Discher, D. E., & Sweeney, H. L. (2006) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.01017.2005 · Cited by 690
Self-citationDivergent Signals and Cytoskeletal Assemblies Regulate Self-Organizing Polarity in Neutrophils
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Cited by
Mechanical forces direct stem cell behaviour in development and regeneration
Vining, K. H., & Mooney, D. J. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.108 · Cited by 1,656
Mechanobiology of collective cell behaviours
Ladoux, B., & Mège, R.-M. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.98 · Cited by 749
FAK controls the mechanical activation of YAP, a transcriptional regulator required for durotaxis
Lachowski, D., Cortes, E., Robinson, B., Rice, A., Rombouts, K., & Del Río Hernández, A. E. (2018) · The FASEB Journal · doi:10.1096/fj.201700721r · Cited by 175
Mechanoaccumulative Elements of the Mammalian Actin Cytoskeleton
Schiffhauer, E. S., Luo, T., Mohan, K., Srivastava, V., Qian, X., Griffis, E. R., Iglesias, P. A., & Robinson, D. N. (2016) · Current Biology · doi:10.1016/j.cub.2016.04.007 · Cited by 108
Gilbert, H. T. J., Mallikarjun, V., Dobre, O., Jackson, M. R., Pedley, R., Gilmore, A. P., Richardson, S. M., & Swift, J. (2019) · Nature Communications · doi:10.1038/s41467-019-11923-1 · Cited by 91
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Metadata
- Authors
- Raab, M., Swift, J., P. Dingal, P. C. D., Shah, P., Shin, J.-W., & Discher, D. E.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2012-11-05
- Volume
- 199
- Issue
- 4
- Pages
- 669-683
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2108832165
- Cited by
- 285
- References
- 43
- FWCI
- 10.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Raab, M., Swift, J., P. Dingal, P. C. D., Shah, P., Shin, J.-W., & Discher, D. E. (2012). Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin-II heavy chain. Journal of Cell Biology. https://doi.org/10.1083/jcb.201205056
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