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A NEW STAINING METHOD (OTO) FOR ENHANCING CONTRAST OF LIPID-CONTAINING MEMBRANES AND DROPLETS IN OSMIUM TETROXIDE-FIXED TISSUE WITH OSMIOPHILIC THIOCARBOHYDRAZIDE (TCH)
Open accessAbstract
Although the introduction of fixation of tissue with osmium tetroxide by Palade (8) ushered in the modern era of electron microscopy of biological materials, a need for greater contrast and greater resolution in visualizing membranous structures has stimulated the introduction of methods for staining with heavy metal salts, in order to enhance the delineation of the fine architecture of cells. This is especially necessary because of the poor contrast of osmium tetroxide-fixed tissue when the epoxy resins are used as embedding materials (9). These methods have relied upon specific affinities of heavy metal salts for various macromolecular components of the cell such as proteins, polysaccharides, nucleoproteins, or lipoproteins (3, 9,
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Metadata
- Authors
- Seligman, A. M., Wasserkrug, H. L., & Hanker, J. S.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1966-08-01
- Volume
- 30
- Issue
- 2
- Pages
- 424-432
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2053341730
- Cited by
- 337
- References
- 0
- FWCI
- 5.93
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A NEW STAINING METHOD (OTO) FOR ENHANCING CONTRAST OF LIPID-CONTAINING MEMBRANES AND DROPLETS IN OSMIUM TETROXIDE-FIXED TISSUE WITH OSMIOPHILIC THIOCARBOHYDRAZIDE (TCH). https://app.cochat.ai/papers/10.1083/jcb.30.2.424
- The paper
- Seligman, A. M., Wasserkrug, H. L., & Hanker, J. S. (1966). A NEW STAINING METHOD (OTO) FOR ENHANCING CONTRAST OF LIPID-CONTAINING MEMBRANES AND DROPLETS IN OSMIUM TETROXIDE-FIXED TISSUE WITH OSMIOPHILIC THIOCARBOHYDRAZIDE (TCH). The Journal of Cell Biology. https://doi.org/10.1083/jcb.30.2.424
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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