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THE MECHANISM OF ACTION OF COLCHICINE
Open accessAbstract
The majority of the colchicine-3H bound by tissue culture cells (KB or Hela) was found to be present as a noncovalent complex with a macromolecule which appears in the soluble fraction after homogenization. Similar binding was demonstrated in vitro and was confined to a component of the soluble fraction. The binding-equilibrium constant and the kinetic constants were essentially the same in vivo and in vitro. Bound radioactivity was reisolated and shown to be present in a molecule with the same chromatographic behavior and specific antimitotic activity as colchicine. In vitro assay of binding activity of a variety of cells and tissues showed a correlation with the presence of microtubules. High binding activity was given by dividing cells, mitotic apparatus, cilia, sperm tails, and brain tissue. Binding to extracts of slime mold or to purified muscle proteins was very low or undetectable. The binding site had a sedimentation constant of 6S and it is suggested that the protein is a subunit of microtubules.
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Cited by
Kapoor, T. M., Mayer, T. U., Coughlin, M. L., & Mitchison, T. J. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.150.5.975 · Cited by 717
ISOLATION OF A PROTEIN SUBUNIT FROM MICROTUBULES
Shelanski, M. L., & Taylor, E. W. (1967) · The Journal of Cell Biology · doi:10.1083/jcb.34.2.549 · Cited by 323
Self-citationStructural Principles of Tau and the Paired Helical Filaments of Alzheimer's Disease
Mandelkow, E., Von Bergen, M., Biernat, J., & Mandelkow, E. (2007) · Brain Pathology · doi:10.1111/j.1750-3639.2007.00053.x · Cited by 243
Information processing in microtubules
Hameroff, S. R., & Watt, R. C. (1982) · Journal of Theoretical Biology · doi:10.1016/0022-5193(82)90137-0 · Cited by 217
Microtubule structure by cryo-EM: snapshots of dynamic instability
Manka, S. W., & Moores, C. A. (2018) · Essays in Biochemistry · doi:10.1042/ebc20180031 · Cited by 92
Metadata
- Authors
- Borisy, G. G., & Taylor, E. W.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1967-08-01
- Volume
- 34
- Issue
- 2
- Pages
- 525-533
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2008190738
- Cited by
- 1,060
- References
- 0
- FWCI
- 31.68
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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