The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
ISOLATION OF A PROTEIN SUBUNIT FROM MICROTUBULES
Open accessAbstract
Sea-urchin sperm tails (Strongylocentrotus purpuratus) were obtained by amputation in synthetic sea water and were purified by differential centrifugation. Most of the arms of the outer nine doublets and soluble matrix proteins were removed by this treatment. The central pairs of microtubules were dissolved by dialysis against EDTA at pH 7.5. The extract contained essentially a single component, with a sedimentation constant of 6S, in amounts sufficient to account for the protein content of the central pairs. Incubation of the extract with colchicine-3H gave binding levels approaching 0.5–1.0 mole of colchicine per 105 g protein. Sucrose-gradient analysis showed that the bound-radioactivity profile coincided with the optical-density profile of the 6S protein. It is concluded that the 6S colchicine-binding protein is a subunit of microtubules.
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Equilibrium Ultracentrifugation of Dilute Solutions*
Yphantis, D. A. (1964) · Biochemistry · doi:10.1021/bi00891a003 · Cited by 3,384
THE MECHANISM OF ACTION OF COLCHICINE
Borisy, G. G., & Taylor, E. W. (1967) · The Journal of Cell Biology · doi:10.1083/jcb.34.2.525 · Cited by 1,060
Self-citationOn Flagellar Structure in Certain Flagellates
Gibbons, I. R., & Grimstone, A. V. (1960) · The Journal of Cell Biology · doi:10.1083/jcb.7.4.697 · Cited by 750
THE MECHANISM OF ACTION OF COLCHICINE
Borisy, G. G., & Taylor, E. W. (1967) · The Journal of Cell Biology · doi:10.1083/jcb.34.2.535 · Cited by 548
Self-citationSTUDIES ON THE PROTEIN COMPONENTS OF CILIA FROM TETRAHYMENA PYRIFORMIS
Gibbons, I. R. (1963) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.50.5.1002 · Cited by 290
Studies on sulfhydryl groups during cell division of sea-urchin eggs
Sakai, H. (1966) · Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis · doi:10.1016/s0926-6585(96)90015-1 · Cited by 114
Cited by
Kapoor, T. M., Mayer, T. U., Coughlin, M. L., & Mitchison, T. J. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.150.5.975 · Cited by 717
Small molecule modulators of protein-protein interactions: selected case studies
Aeluri, M., Chamakuri, S., Dasari, B., Guduru, S. K. R., Jimmidi, R., Jogula, S., & Arya, P. (2014) · Chemical Reviews · doi:10.1021/cr4004049 · Cited by 79
All Wired Up: An Exploration of the Electrical Properties of Microtubules and Tubulin
Kalra, A. P., Eakins, B. B., Patel, S. D., Ciniero, G., Rezania, V., Shankar, K., & Tuszynski, J. A. (2020) · ACS Nano · doi:10.1021/acsnano.0c06945 · Cited by 49
Metadata
- Authors
- Shelanski, M. L., & Taylor, E. W.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1967-08-01
- Volume
- 34
- Issue
- 2
- Pages
- 549-554
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2055909932
- Cited by
- 323
- References
- 7
- FWCI
- 21.41
- Type
- journal article
- Language
- en
- Open access
- green
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- Shelanski, M. L., & Taylor, E. W. (1967). ISOLATION OF A PROTEIN SUBUNIT FROM MICROTUBULES. The Journal of Cell Biology. https://doi.org/10.1083/jcb.34.2.549
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