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Cited by 559

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATE

Open access
Hirsch, James G., Fedorko, Martha E.·1968·The Journal of Cell Biology

Abstract

Human leukocytes in suspension or in monolayer cultures have been processed for electron microscopy by fixation in a freshly made cold mixture of glutaraldehyde and osmium tetroxide and by "postfixation" in uranyl acetate. Simultaneous exposure to glutaraldehyde and osmium tetroxide eliminates many of the shortcomings seen when either of these agents is used alone as the initial fixative. Specimens are processed to the stage of dehydration as single cell suspensions or as very small clumps to assure rapid penetration of fixatives and efficient washing. The technique is rapid and reproducible. Electron micrographs presented in this report illustrate the ultrastructural features of human white cells prepared by this method.

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Crossref · 10.1083/jcb.38.3.615

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Metadata

Authors
Hirsch, J. G., & Fedorko, M. E.
Venue
The Journal of Cell Biology
Published
1968-09-01
Volume
38
Issue
3
Pages
615-627
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W2067184583
Cited by
559
References
1
FWCI
29.38
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATE. https://app.cochat.ai/papers/10.1083/jcb.38.3.615
The paper
Hirsch, J. G., & Fedorko, M. E. (1968). ULTRASTRUCTURE OF HUMAN LEUKOCYTES AFTER SIMULTANEOUS FIXATION WITH GLUTARALDEHYDE AND OSMIUM TETROXIDE AND "POSTFIXATION" IN URANYL ACETATE. The Journal of Cell Biology. https://doi.org/10.1083/jcb.38.3.615

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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