Unusually high mitochondrial alpha glycerophosphate dehydrogenase activity in rat brown adipose tissue
Open accessAbstract
Brown adipose tissue of the rat has been found to have an unusually high activity of mitohondrial alpha-glycerophosphate dehydrogenase (alpha-GPD) when assayed both by a histochemical staining procedure and by a quantitative biochemical method with isolated mitochondria. In contrast to succinic, glutamic, and beta-hydroxybutyrate dehydrogenases, all mitochondrial enzymes, the activity of alpha-GPD in brown fat was 10 times that in liver, more than 20 times that in white adipose tissue, and 9 times that in kidney. The soluble NAD-linked alpha-GPD was also higher in brown adipose tissue than in white adipose tissue, liver, or kidney, but the differences were much less marked. The possible importance of the high activity of mitochondrial alpha-GPD in the regulation of synthesis of esterified lipid and in thermogenesis in brown fat is discussed.
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The glycerol 3-phosphate shuttle is not considered the major route of NADH shuttling in most mammalian cells but is particularly active in brown adipose tissue and may be implicated in thermoregulation.
“The glycerol 3-phosphate shuttle is not considered the major route of NADH shuttling in most mammalian cells but has been shown to be particularly active in brown adipose tissue and may be implicated in thermoregulation (105, 106, 107).”
From Regulation and function of the mammalian tricarboxylic acid cycle (2023) · cites this paper as [106]
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- Authors
- Ohkawa, K.-I., Vogt, M. T., & Farber, E.
- Affiliations
- University of Pittsburgh (US)
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1969-05-01
- Volume
- 41
- Issue
- 2
- Pages
- 441-449
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2107766
- OpenAlex
- W2051900167
- Cited by
- 47
- References
- 19
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2019: 3 · 2017: 2 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 2.11
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- alpha-glycerophosphate dehydrogenase, brown adipose tissue, mitochondrial enzymes, enzyme activity, thermogenesis, white adipose tissue, liver, kidney, rats
- MeSH terms
- Adipose Tissue, Animals, Adipose Tissue, Brown, Glutamate Dehydrogenase, Glycerolphosphate Dehydrogenase, Histocytochemistry, Hydroxybutyrate Dehydrogenase, Kidney, Liver, Male, Mitochondria, Succinate Dehydrogenase, Rats
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