The DOI matches its Crossref record.
BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF AZUROPHIL AND SPECIFIC GRANULES OF HUMAN NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES
Open accessAbstract
Postnuclear supernates from homogenates of purified neutrophil polymorphonuclear leukocytes (PMNs) from human blood were fractionated by zonal sedimentation and isopycnic equilibration in sucrose gradients. The fractions were characterized biochemically by measuring protein content and the activities of eight enzymes. Selected fractions were further analyzed by electron microscopy. In both centrifugation systems, azurophil and specific granules could be resolved almost completely. Azurophil granules sediment three to four times faster than the specifics and have an average density of 1.23. They contain all the peroxidase of the cells, large portions of four lysosomal hydrolases, and about half of the total lysozyme, and therefore appear to be, in biochemical terms, very similar to the azurophil granules of rabbit PMNs. The specific granules, which have an average density of 1.19, contain the remaining half of the lysozyme but appear to be free of the other components of the azurophil granules, and of alkaline phosphatase. Isopycnic equilibration disclosed a minor lysosomal population, which strongly overlaps the specific granules, and made possible the identification of a membrane-fraction which is characterized by the presence of the thiol-sensitive acid 4-nitrophenyl phosphatase and of alkaline phosphatase.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Heyworth, P. G., Cross, A. R., & Curnutte, J. T. (2003) · Current Opinion in Immunology · doi:10.1016/s0952-7915(03)00109-2 · Cited by 396
Human neutrophil granules and secretory vesicles
Borregaard, N., Lollike, K., Kjeldsen, L., Sengeløv, H., Bastholm, L., Nielsen, M. H., & Bainton, D. F. (1993) · European Journal Of Haematology · doi:10.1111/j.1600-0609.1993.tb00629.x · Cited by 295
Release of Gelatinase from a Novel Secretory Compartment of Human Neutrophils
Dewald, B., Bretz, U., & Baggiolini, M. (1982) · Journal of Clinical Investigation · doi:10.1172/jci110643 · Cited by 263
Self-citationPeterson, C. G., Eklund, E., Taha, Y., Raab, Y., & Carlson, M. (2002) · American Journal of Gastroenterology · doi:10.1111/j.1572-0241.2002.05837.x · Cited by 137
Metadata
- Authors
- Bretz, U., & Baggiolini, M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1974-10-01
- Volume
- 63
- Issue
- 1
- Pages
- 251-269
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2026721162
- Cited by
- 419
- References
- 0
- FWCI
- 4.11
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF AZUROPHIL AND SPECIFIC GRANULES OF HUMAN NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES. https://app.cochat.ai/papers/10.1083/jcb.63.1.251
- The paper
- Bretz, U., & Baggiolini, M. (1974). BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF AZUROPHIL AND SPECIFIC GRANULES OF HUMAN NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES. The Journal of Cell Biology. https://doi.org/10.1083/jcb.63.1.251
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
