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A New Method for The Quantification of Neutrophil and Eosinophil Cationic Proteins in Feces: Establishment of Normal Levels and Clinical Application in Patients With Inflammatory Bowel Disease

Peterson, Christer GB, Eklund, Elisabeth, Taha, Yesuf, Raab, Yngve, Carlson, Marie·2002·American Journal of Gastroenterology
DOI 10.1111/j.1572-0241.2002.05837.x
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Abstract

Objectives The aims of this study were 1) to develop a valid method for the measurement of the eosinophil proteins eosinophil cationic protein (ECP) and eosinophil protein X (EPX) and neutrophil proteins myeloperoxidase and human neutrophil lipocalin (HNL) in feces and 2) to investigate their potential role as disease activity markers in inflammatory bowel disease (IBD). Methods Feces samples were obtained from 44 apparently healthy individuals (HIs), 18 patients with IBD (11 with ulcerative colitis [UC] and seven with Crohn's disease [CD]), and three with collagen colitis. The granulocyte markers were measured using immunoassays in supernatants from processed feces. Results ECP, myeloperoxidase, and, to a lesser degree, EPX and HNL were bound to the solid part of feces. However, feces homogenized in an extraction buffer containing the cationic detergent N-cetyl-N,N,N-trimethylammonium bromide allowed an efficient recovery of the proteins (i.e., up to 100-fold increased levels compared to homogenization in saline). All four proteins were stable for at least 7 days at +6 degrees C and at least 3 days at +22 degrees C. The normal fecal geometric mean (95th percentile) levels of ECP, EPX, myeloperoxidase, and HNL were estimated to be, respectively, 1.69 microg/g (6.41), 0.57 microg/g (1.72), 3.54 microg/g (8.77), and 1.97 microg/g (4.91). Markedly increased feces levels of all markers (p Conclusions The method described here takes into account the molecular properties of the gra…

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Research works printed but not linked: 34

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Metadata

Authors
Peterson, C. G., Eklund, E., Taha, Y., Raab, Y., & Carlson, M.
Venue
American Journal of Gastroenterology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2002-07
Volume
97
Issue
7
Pages
1755-1762
ISSN
0002-9270
DOI
10.1111/j.1572-0241.2002.05837.x
Cited by
137
References
0
Language
en

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